Clostridium aminophilum FT: Difference between revisions
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== Genome == | == Genome == | ||
The genome of ''C. aminophilum'' FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka. | The genome of ''C. aminophilum'' FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka. | ||
[[File:Cami genome.jpg|alt=A representation of the C. aminophilum FT genome.|center|''C. aminophilum'' F<sup>T</sup> genome with tRNA, rRNA, and CRISPR loci | [[File:Cami genome.jpg|alt=A representation of the C. aminophilum FT genome.|center|''C. aminophilum'' F<sup>T</sup> genome with tRNA, rRNA, and CRISPR loci annotated. Source: Dr. William G. Alexander|frame]] | ||
== Methylome == | == Methylome == | ||
| Line 56: | Line 57: | ||
== Culturing Conditions == | == Culturing Conditions == | ||
{| class="wikitable" | |||
|+ | |||
|Media | |||
|[[Reinforced Clostridial Medium|RCM]] (+cysteine) + [[Wolin's Vitamin Solution|Wolin's]] | |||
|RCM (+cysteine) +[[Wolin's Vitamin Solution|Wolin's]] +[[Volatile Fatty Acid Solution|VFA]] | |||
|RCM +cysteine +[[Wolin's Vitamin Solution|Wolin's]] +[[Volatile Fatty Acid Solution|VFA]] +TM | |||
|M2GSC | |||
|MDM | |||
|- | |||
|Time to Saturation | |||
|one overnight period | |||
|one overnight period | |||
|one overnight period | |||
|one overnight period | |||
|Not tested | |||
|} | |||
== Antibiotic MIC Tests == | == Antibiotic MIC Tests == | ||
[[File:Cami MIC.jpg|left|thumb|Raw photographic data of MIC tests on ''C. aminophilum'' FT]] | [[File:Cami MIC.jpg|left|thumb|Raw photographic data of MIC tests on ''C. aminophilum'' FT. Source: Dr. Jill Walton]] | ||
{| class="wikitable sortable mw-collapsible mw-collapsed" | {| class="wikitable sortable mw-collapsible mw-collapsed" | ||
!Antibiotic | !Antibiotic | ||
Latest revision as of 18:41, 14 October 2026
Global Methane Hub Metadata
Genome
The genome of C. aminophilum FT consists of a single, circular contig 3.2 Mbp in size. Seven rRNA loci (each with three rRNA genes), 60 tRNA loci, and 2 CRISPR loci were annotated by Prokka.
Methylome
| motif | # sites methylated | total sites | % methylated |
|---|---|---|---|
| G(6mA)TCNNNNNGGT | 1057 | 1060 | 99.72 |
| (6mA)CCNNNNNGATC | 1018 | 1060 | 96.04 |
| GCC(6mA)NNNNNNNNTGT | 191 | 197 | 96.45 |
| AC(6mA)NNNNNNNNTGGC | 186 | 197 | 94.42 |
| CG(6mA)CNNNNNNTCG | 191 | 191 | 100 |
| CG(6mA)NNNNNNGTCG | 190 | 191 | 99.48 |
| ATTA(6mA)T | 440 | 466 | 94.42 |
| C(5mC)GCGG | 3276 | 3450 | 94.96 |
Methylating E. coli strains
Culturing Conditions
| Media | RCM (+cysteine) + Wolin's | RCM (+cysteine) +Wolin's +VFA | RCM +cysteine +Wolin's +VFA +TM | M2GSC | MDM |
| Time to Saturation | one overnight period | one overnight period | one overnight period | one overnight period | Not tested |
Antibiotic MIC Tests
| Antibiotic | Concentration Range |
|---|---|
| Tetracycline | 12-16 ug/mL |
| Trimethoprim | Resistant |
| Ampicillin | 0.25-0.38 ug/mL |
| Streptomycin | Resistant |
| Chloramphenicol | 8-12 ug/mL |
| Gentamycin | 32-48 ug/mL |
| Kanamycin | 128-192 ug/mL |
| Erythromycin | 0.094-0.19 ug/mL |
| Spectinomycin | Resistant |
