Volatile Fatty Acid Solution: Difference between revisions
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Alexanderwg (talk | contribs) Created page with "== Description == This Volatile Fatty Acid (VFA) solution is a supplement used in a variety of anaerobic media formulations. == Recipe == == Used With == * Reinforced Clostridium Media (RCM)" |
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== Recipe == | == Recipe == | ||
{| class="wikitable" | |||
|+ | |||
!Component | |||
!Volume for 5X Stock Solution | |||
|- | |||
|deionized water | |||
|30 mL | |||
|- | |||
|isobutyric acid | |||
|1 mL | |||
|- | |||
|n-valeric acid | |||
|1 mL | |||
|- | |||
|isovaleric acid | |||
|1 mL | |||
|- | |||
|DL-α-methylbutyric acid | |||
|1 mL | |||
|} | |||
Add to a mixing vessel sequentially, pH to 7.0 with 1 M sodium hydroxide, then bring to a final volume of 100 mL with water. Filter sterilize and store at -20 °C. | |||
== Used With == | == Used With == | ||
* [[Reinforced Clostridium Media|Reinforced Clostridium Media (RCM)]] | * [[Reinforced Clostridium Media|Reinforced Clostridium Media (RCM)]] | ||
Latest revision as of 19:36, 23 July 2026
Description
This Volatile Fatty Acid (VFA) solution is a supplement used in a variety of anaerobic media formulations.
Recipe
| Component | Volume for 5X Stock Solution |
|---|---|
| deionized water | 30 mL |
| isobutyric acid | 1 mL |
| n-valeric acid | 1 mL |
| isovaleric acid | 1 mL |
| DL-α-methylbutyric acid | 1 mL |
Add to a mixing vessel sequentially, pH to 7.0 with 1 M sodium hydroxide, then bring to a final volume of 100 mL with water. Filter sterilize and store at -20 °C.
