Aristaeella hokkaidonensis R-7T: Difference between revisions
Mahoneykurpe (talk | contribs) Created page with " == Introduction == == Global Methane Hub Metadata == {| class="wikitable" |+ |Origin: | |- |Sequenced by: | |- |Assembled by: | |- |Methylome called by: | |- |Methyltransferases cloned by: | |- |MIC and Culturing Conditions by: | |- |Transformation by: | |} == Genome == == DefenseFinder Results == == Methylome == == Methylating E. coli Strains == == Culturing Conditions == == Antibiotic MIC Tests == == Protocols == == Transformation Results == == External Link..." |
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== Introduction == | == Introduction == | ||
''Aristaeella hokkaidonensis'' R-7<sup>T</sup> (DSM 112795, JCM 34733) was isolated from serial dilutions of the cellulose-adherent fraction from incubations of crystalline cellulose with rumen contents collected from a sheep fed a 9:1 forage-concentrate diet in Hokkaido, Japan. R-7 ferments a range of substrates including the plant cell wall polysaccharides xylan and pectin, though despite its apparent adherence, not crystalline cellulose. R-7 forms acetate, hydrogen, ethanol, and lactate as fermentation end products [1]. | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
| Line 28: | Line 29: | ||
== Genome == | == Genome == | ||
{| class="wikitable" | |||
|+ | |||
! colspan="2" |Genome characteristics | |||
|- | |||
|Contigs | |||
|1 | |||
|- | |||
|size (bp) | |||
|3,390,520 | |||
|- | |||
|GC % | |||
|53.0 | |||
|- | |||
|CDS | |||
|2966 | |||
|- | |||
|rRNA | |||
|9 | |||
|- | |||
|tRNA | |||
|49 | |||
|- | |||
|CRISPR | |||
|0 | |||
|} | |||
== DefenseFinder Results == | == DefenseFinder Results == | ||
== Methylome == | == Methylome == | ||
{| class="wikitable" | |||
|+ | |||
!Methylated motif | |||
!Percent methylated | |||
|- | |||
|(6mA)CGNNNNNTGC | |||
|98.0 | |||
|- | |||
|GC(6mA)NNNNNCGT | |||
|92.7 | |||
|- | |||
|TGG(6mA)NNNNNNTRC | |||
|99.8 | |||
|- | |||
|GY(6mA)NNNNNNTCCA | |||
|93.3 | |||
|- | |||
|CCAAA(6mA) | |||
|97.1 | |||
|} | |||
== Methylating E. coli Strains == | == Methylating E. coli Strains == | ||
| Line 44: | Line 90: | ||
== External Links == | == External Links == | ||
[1] Mahoney-Kurpe SC, Palevich N, Noel SJ, Gagic D, Biggs PJ, Soni P, Reid PM, Koike S, Kobayashi Y, Janssen PH, Attwood GT, Moon CD. 2023. ''Aristaeella hokkaidonensis'' gen. nov. sp. nov. and ''Aristaeella lactis'' sp. nov., two rumen bacterial species of a novel proposed family, ''Aristaeellaceae'' fam. nov. Int J Syst Evol Microbiol 73:005831. | |||
Latest revision as of 20:30, 8 November 2026
Introduction
Aristaeella hokkaidonensis R-7T (DSM 112795, JCM 34733) was isolated from serial dilutions of the cellulose-adherent fraction from incubations of crystalline cellulose with rumen contents collected from a sheep fed a 9:1 forage-concentrate diet in Hokkaido, Japan. R-7 ferments a range of substrates including the plant cell wall polysaccharides xylan and pectin, though despite its apparent adherence, not crystalline cellulose. R-7 forms acetate, hydrogen, ethanol, and lactate as fermentation end products [1].
Global Methane Hub Metadata
| Origin: | |
| Sequenced by: | |
| Assembled by: | |
| Methylome called by: | |
| Methyltransferases cloned by: | |
| MIC and Culturing Conditions by: | |
| Transformation by: |
Genome
| Genome characteristics | |
|---|---|
| Contigs | 1 |
| size (bp) | 3,390,520 |
| GC % | 53.0 |
| CDS | 2966 |
| rRNA | 9 |
| tRNA | 49 |
| CRISPR | 0 |
DefenseFinder Results
Methylome
| Methylated motif | Percent methylated |
|---|---|
| (6mA)CGNNNNNTGC | 98.0 |
| GC(6mA)NNNNNCGT | 92.7 |
| TGG(6mA)NNNNNNTRC | 99.8 |
| GY(6mA)NNNNNNTCCA | 93.3 |
| CCAAA(6mA) | 97.1 |
Methylating E. coli Strains
Culturing Conditions
Antibiotic MIC Tests
Protocols
Transformation Results
External Links
[1] Mahoney-Kurpe SC, Palevich N, Noel SJ, Gagic D, Biggs PJ, Soni P, Reid PM, Koike S, Kobayashi Y, Janssen PH, Attwood GT, Moon CD. 2023. Aristaeella hokkaidonensis gen. nov. sp. nov. and Aristaeella lactis sp. nov., two rumen bacterial species of a novel proposed family, Aristaeellaceae fam. nov. Int J Syst Evol Microbiol 73:005831.
