Selenomonas ruminantium PC18: Difference between revisions
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== Introduction == | == Introduction == | ||
'''Pseudoselenomonas lactilytica PC 18<sup>T</sup>''' | |||
Pseudoselenomonas lactilytica PC18<sup>T</sup> (DSM 2872), formerly Selenomonas ruminantium subsp. lactilytica was isolated from bovine rumen contents from an animal fed a grass pasture and grain mixture in the USA [1]. PC 18 is an anaerobic bacterium that ferments a broad range of carbohydrates and produces primarily propionate, lactate, and CO₂ [1, 2]. Lactate is fermented to propionate and acetate [1], and PC 18 can reduce nitrate, sulfate and fumarate. PC 18 is the type strain of the species [3]. | |||
Rumen metatranscriptomic and metagenomic studies have shown that in addition to methanogenesis there are other pathways of H<sub>2</sub> use, including nitrate, sulfate, and fumarate reduction as well as homoacetogenesis [4]. Sulfate and nitrate are external electron acceptors that can be taken up from the environment and reduced to sulfide and ammonia, respectively, while fumarate is reduced to succinate followed by decarboxylation to propionate. | |||
The draft genome for PC 18 (3,039,002 bp) was generated through the Hungate1000 project with JGI [5]. Resequencing gave a 3,074,662 genome consisting of a chromosome (2,979,598 bp) and two plasmids (76,524 bp and 18,540 bp). | |||
LPSN: Pseudoselenomonas lactilytica (Bryant 1956) Heng and Kittelmann 2026. | |||
NCBI: Pseudoselenomonas lactilytica. | |||
GTDB: Selenomonas_A ruminantium_B. | |||
Risk group: 1. | |||
== Global Methane Hub Metadata == | == Global Methane Hub Metadata == | ||
| Line 25: | Line 40: | ||
== Genome == | == Genome == | ||
== DefenseFinder Results == | |||
== Methylome == | |||
== Methylating E. coli Strains == | |||
''E. coli'' strain AG22287 has been developed by ORNL to match the methylome of PC 18. | |||
== Culturing Conditions == | |||
PC 18 grows very well in M2G medium, reaching OD >2.5 within ~3-5 h. PC 18 also grows well on M2G agar plates, with a plating efficiency of ~70%. | |||
== Antibiotic MIC Tests == | |||
{| class="wikitable" | |||
|+ | |||
!antibiotic | |||
!concentration (μg/ml) | |||
|- | |||
|carbenicillin | |||
|5 | |||
|- | |||
|clarithromycin | |||
|50 | |||
|- | |||
|tetracycline | |||
|insensitive | |||
|- | |||
|thiamphenicol | |||
|1 | |||
|} | |||
== Protocols == | |||
== Transformation Results == | |||
== External Links == | |||
[1] Bryant MP. 1956. The characteristics of strains of Selenomonas isolated from bovine rumen contents. J Bacteriol 72: 162-7. doi: 10.1128/jb.72.2.162-167.1956. | |||
[2] <nowiki>https://bacdive.dsmz.de/strain/17155</nowiki>. | |||
[3] Heng YC, Kittelmann S. 2026. Taxonomic reclassification and expansion of the family Selenomonadaceae. Int J Syst Evol Microbiol 76: 7178. doi: 10.1099/ijsem.0.007178. | |||
[4] Greening C, Geier R, Wang C, Woods LC, Morales SE, McDonald MJ, Rushton-Green R, Morgan XC, Koike S, Leahy SC, Kelly WJ, Cann I, Attwood GT, Cook GM, Mackie RI. 2019. Diverse hydrogen production and consumption pathways influence methane production in ruminants. ISME J 13: 2617-2632. doi: 10.1038/s41396-019-0464-2. | |||
[5] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110. | |||
Latest revision as of 04:22, 2 November 2026
Introduction
Pseudoselenomonas lactilytica PC 18T
Pseudoselenomonas lactilytica PC18T (DSM 2872), formerly Selenomonas ruminantium subsp. lactilytica was isolated from bovine rumen contents from an animal fed a grass pasture and grain mixture in the USA [1]. PC 18 is an anaerobic bacterium that ferments a broad range of carbohydrates and produces primarily propionate, lactate, and CO₂ [1, 2]. Lactate is fermented to propionate and acetate [1], and PC 18 can reduce nitrate, sulfate and fumarate. PC 18 is the type strain of the species [3].
Rumen metatranscriptomic and metagenomic studies have shown that in addition to methanogenesis there are other pathways of H2 use, including nitrate, sulfate, and fumarate reduction as well as homoacetogenesis [4]. Sulfate and nitrate are external electron acceptors that can be taken up from the environment and reduced to sulfide and ammonia, respectively, while fumarate is reduced to succinate followed by decarboxylation to propionate.
The draft genome for PC 18 (3,039,002 bp) was generated through the Hungate1000 project with JGI [5]. Resequencing gave a 3,074,662 genome consisting of a chromosome (2,979,598 bp) and two plasmids (76,524 bp and 18,540 bp).
LPSN: Pseudoselenomonas lactilytica (Bryant 1956) Heng and Kittelmann 2026.
NCBI: Pseudoselenomonas lactilytica.
GTDB: Selenomonas_A ruminantium_B.
Risk group: 1.
Global Methane Hub Metadata
| Origin | |
| Sequenced by | |
| Assembled by | |
| Methylome called by | |
| MIC and culturing conditions by | |
| Transformation by |
Genome
DefenseFinder Results
Methylome
Methylating E. coli Strains
E. coli strain AG22287 has been developed by ORNL to match the methylome of PC 18.
Culturing Conditions
PC 18 grows very well in M2G medium, reaching OD >2.5 within ~3-5 h. PC 18 also grows well on M2G agar plates, with a plating efficiency of ~70%.
Antibiotic MIC Tests
| antibiotic | concentration (μg/ml) |
|---|---|
| carbenicillin | 5 |
| clarithromycin | 50 |
| tetracycline | insensitive |
| thiamphenicol | 1 |
Protocols
Transformation Results
External Links
[1] Bryant MP. 1956. The characteristics of strains of Selenomonas isolated from bovine rumen contents. J Bacteriol 72: 162-7. doi: 10.1128/jb.72.2.162-167.1956.
[2] https://bacdive.dsmz.de/strain/17155.
[3] Heng YC, Kittelmann S. 2026. Taxonomic reclassification and expansion of the family Selenomonadaceae. Int J Syst Evol Microbiol 76: 7178. doi: 10.1099/ijsem.0.007178.
[4] Greening C, Geier R, Wang C, Woods LC, Morales SE, McDonald MJ, Rushton-Green R, Morgan XC, Koike S, Leahy SC, Kelly WJ, Cann I, Attwood GT, Cook GM, Mackie RI. 2019. Diverse hydrogen production and consumption pathways influence methane production in ruminants. ISME J 13: 2617-2632. doi: 10.1038/s41396-019-0464-2.
[5] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.
