Eubacterium maltosivorans SA11 transformation protocol (BSI): Revision history

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30 October 2026

  • curprev 08:0008:00, 30 October 2026 Mahoneykurpe talk contribs 2,312 bytes +2,312 Created page with "Based on protocol for ''E. limosum'' described in Sanford & Woolston (2022) [1], with some adjustments made based on results from initial electroporation tests which gave a transformation efficiency of approx. 6,000 CFU/µg in a best attempt. === '''Electrocompetent cell preparation''' === # Grow six 10 ml SA11 cultures (or upscale as required) to early log phase (OD ~0.5) # Cool cultures on ice for 20 min. # Bring cultures into chamber on ice, pour 30 ml culture into..." Tag: Visual edit
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