Streptococcus equinus B315 - Revision history

Ahetta at 18:47, 29 October 2026

2026-10-29T18:47:24Z

← Older revision		Revision as of 18:47, 29 October 2026
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	\|-		\|-
	\|Ampicillin		\|Ampicillin
	\|0.25		\|0.44
	\|-		\|-
	\|Kanamycin		\|Kanamycin
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	\|-		\|-
	\|Chloramphenicol		\|Chloramphenicol
	\|0.50		\|0.49
	\|-		\|-
	\|Tetracycline		\|Tetracycline
	\|~~0.23~~		\|1
	\|-		\|-
	\|Gentamicin		\|Gentamicin
	\|1.0		\|11.1
	\|-		\|-
	\|Streptomycin		\|Streptomycin

Ahetta at 18:39, 29 October 2026

2026-10-29T18:39:18Z

← Older revision		Revision as of 18:39, 29 October 2026
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	== Protocols ==		== Protocols ==

			* '''[[SDM culture medium\|Preparation of SDM culture medium]]'''
			* '''Preparation of M2GSC culture medium'''

			* [[Streptococcus transformation protocol\|'''Transformation protocol''']]

	== Transformation Results ==		== Transformation Results ==

Ahetta at 17:06, 29 October 2026

2026-10-29T17:06:52Z

@@ Line 70: / Line 70: @@
 == Culturing Conditions ==
 == Antibiotic MIC Tests ==
 == Protocols ==

Ahetta: /* Methylome */

2026-10-29T17:00:16Z

Methylome

← Older revision		Revision as of 17:00, 29 October 2026
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	== Methylome ==		== Methylome ==
			{\| class="wikitable"
			\| valign="top" \|'''#6mA modified motifs'''
			\| valign="top" \|
			\| valign="top" \|
			\| valign="top" \|
			\|-
			\| valign="top" \|'''#motif'''
			\| valign="top" \|methylCounts
			\| valign="top" \|totalCounts
			\| valign="top" \|%motifsMethyl
			\|-
			\| valign="top" \|'''GAR(6mA)NNNNNNNTAC'''
			\| valign="top" \|412
			\| valign="top" \|430
			\| valign="top" \|95.81
			\|-
			\| valign="top" \|'''GT(6mA)NNNNNNNTYTC'''
			\| valign="top" \|413
			\| valign="top" \|430
			\| valign="top" \|96.05
			\|}

	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==

Ahetta at 16:56, 29 October 2026

2026-10-29T16:56:06Z

← Older revision		Revision as of 16:56, 29 October 2026
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			[[File:Streptococcus equinus B315 scanning electron micrograph by Ahmed Hetta.jpg\|thumb\|350x350px\|Streptococcus equinus B315 scanning electron micrograph by Ahmed Hetta]]

	== Introduction ==		== Introduction ==

Ahetta: /* Global Methane Hub Metadata */

2026-10-29T16:50:58Z

Global Methane Hub Metadata

← Older revision		Revision as of 16:50, 29 October 2026
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	\|+		\|+
	\|Origin:		\|Origin:
	\|		\|New Zealand
	\|-		\|-
	\|Sequenced by:		\|Sequenced by:
	\|		\|Ahmed Hetta and Yuqi Li
	\|-		\|-
	\|Assembled by:		\|Assembled by:
	\|		\|William Alexander
	\|-		\|-
	\|Methylome called by:		\|Methylome called by:
	\|		\|William Alexander
	\|-		\|-
	\|Methyltransferases cloned by:		\|Methyltransferases cloned by:
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	\|-		\|-
	\|Transformation by:		\|Transformation by:
	\|		\|Ahmed Hetta
	\|}		\|}

Billkelleydonvis: Introduction and external links

2026-10-28T19:51:07Z

Introduction and external links

New page

== Introduction ==
'''''Streptococcus equinus'' B315'''

''Streptococcus equinus'' B315 was isolated from bovine rumen contents in NZ [1]. B315 is proteolytic, Gram positive, and able to ferment a wide range of carbohydrates. Its main fermentation product is lactate, together with succinate and acetate [1]. The B315 genome encodes two lantibiotic bacteriocin biosynthetic gene clusters.

The draft genome for B315 (1,786,673 bp) was generated through the Hungate1000 project with JGI [2].

NCBI: ''Streptococcus equinus''.

GTDB: ''Streptococcus equinus''. B315 belongs to the same species group as the ''S. equinus'' type strain (DSM 20558).

Risk group: 2.

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|
|-
|Sequenced by:
|
|-
|Assembled by:
|
|-
|Methylome called by:
|
|-
|Methyltransferases cloned by:
|
|-
|MIC and Culturing Conditions by:
|
|-
|Transformation by:
|
|}

== Genome ==

== DefenseFinder Results ==

== Methylome ==

== Methylating E. coli Strains ==

== Culturing Conditions ==

== Antibiotic MIC Tests ==

== Protocols ==

== Transformation Results ==

== External Links ==
[1] Attwood GT, Reilly K. 1995. Identification of proteolytic rumen bacteria isolated from New Zealand cattle. J Appl Bacteriol 79: 22-9. doi: 10.1111/j.1365-2672.1995.tb03119.x.

[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

← Older revision		Revision as of 17:00, 29 October 2026
Line 45:		Line 45:

	== Methylome ==		== Methylome ==
			{\| class="wikitable"
			\| valign="top" \|'''#6mA modified motifs'''
			\| valign="top" \|
			\| valign="top" \|
			\| valign="top" \|
			\|-
			\| valign="top" \|'''#motif'''
			\| valign="top" \|methylCounts
			\| valign="top" \|totalCounts
			\| valign="top" \|%motifsMethyl
			\|-
			\| valign="top" \|'''GAR(6mA)NNNNNNNTAC'''
			\| valign="top" \|412
			\| valign="top" \|430
			\| valign="top" \|95.81
			\|-
			\| valign="top" \|'''GT(6mA)NNNNNNNTYTC'''
			\| valign="top" \|413
			\| valign="top" \|430
			\| valign="top" \|96.05
			\|}

	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==