Streptococcus bovis JB1 - Revision history

Ahetta at 17:09, 30 October 2026

2026-10-30T17:09:09Z

← Older revision		Revision as of 17:09, 30 October 2026
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	* [[SDM culture medium\|Preparation of SDM culture medium]]		* [[SDM culture medium\|Preparation of SDM culture medium]]
	* Preparation of M2GSC culture medium		* [[M2GSC Medium\|Preparation of M2GSC culture medium]]

	* [[Streptococcus transformation protocol\|Streptococcus transformation '''protocol''']]		* [[Streptococcus transformation protocol\|Streptococcus transformation '''protocol''']]

Ahetta at 18:43, 29 October 2026

2026-10-29T18:43:05Z

← Older revision		Revision as of 18:43, 29 October 2026
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	== Protocols ==		== Protocols ==

	* ~~'''~~[[SDM culture medium\|Preparation of SDM culture medium]]~~'''~~		* [[SDM culture medium\|Preparation of SDM culture medium]]
	* ~~'''~~Preparation of M2GSC culture medium~~'''~~		* Preparation of M2GSC culture medium

	* [[Streptococcus transformation protocol\|'''~~Transformation~~ protocol''']]		* [[Streptococcus transformation protocol\|Streptococcus transformation '''protocol''']]

	== Transformation Results ==		== Transformation Results ==

Ahetta at 18:41, 29 October 2026

2026-10-29T18:41:31Z

← Older revision		Revision as of 18:41, 29 October 2026
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	== Introduction ==		== Introduction ==
	''Streptococcus bovis'' JB1 was isolated from a cow fed alfalfa hay in Davis, CA, USA~~<sup>~~1~~</sup>~~. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.		''Streptococcus bovis'' JB1 was isolated from a cow fed alfalfa hay in Davis, CA, USA[1]. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.

Ahetta at 16:48, 29 October 2026

2026-10-29T16:48:25Z

← Older revision		Revision as of 16:48, 29 October 2026
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			[[File:S. bovis SEM.jpg\|thumb\|350x350px\|Scanning electron micrograph of ''Streptococcus bovis'' JB1 by Ahmed Hetta]]

	~~[[File:SEM_electron_micrograph_of_Streptococcus_bovis_JB1.jpg\|thumb\|300x300px\|~~''~~S. azabuensis~~'' ~~KH1P5~~ in ~~liquid culture exhibiting its dense layer~~ of ~~EPS. Source: Dr~~. ~~Roy Rodriguez Carrero]]~~		== Introduction ==
			''Streptococcus bovis'' JB1 was isolated from a cow fed alfalfa hay in Davis, CA, USA<sup>1</sup>. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.

	~~== Introduction ==~~
	''Streptococcus bovis'' JB1 was isolated from the rumen of alfalfa-fed cow. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.


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	== External Links ==		== External Links ==
	[
			# arvis GN, Kurtovic A, Hay AG, Russell JB. The physiological and genetic diversity of bovine Streptococcus bovis strains(1). FEMS Microbiol Ecol. 2001 Mar;35(1):49-56. doi: 10.1016/s0168-6496(00)00110-0. PMID: 11248389.

Ahetta at 01:15, 29 October 2026

2026-10-29T01:15:11Z

← Older revision		Revision as of 01:15, 29 October 2026
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	== External Links ==		== External Links ==
	[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.		[

	[2] Kumar S, Treloar BP, Teh KH, McKenzie CM, Henderson G, Attwood GT, Waters SM, Patchett ML, Janssen PH. 2018. Sharpea and Kandleria are lactic acid producing rumen bacteria that do not change their fermentation products when co-cultured with a methanogen. Anaerobe 54: 31-38. doi: 10.1016/j.anaerobe.2018.07.008.

	[3] Kittelmann S, Pinares-Patiño CS, Seedorf H, Kirk MR, Ganesh S, McEwan JC, Janssen PH. 2014. Two different bacterial community types are linked with the low-methane emission trait in sheep. PLoS One 9: e103171. doi: 10.1371/journal.pone.0103171.

	[4] Kamke J, Kittelmann S, Soni P, Li Y, Tavendale M, Ganesh S, Janssen PH, Shi W, Froula J, Rubin EM, Attwood GT. 2016. Rumen metagenome and metatranscriptome analyses of low methane yield sheep reveals a Sharpea-enriched microbiome characterised by lactic acid formation and utilisation. Microbiome 4: 56. doi: 10.1186/s40168-016-0201-2.

	[5] Morita H, Shiratori C, Murakami M, Takami H, Toh H, Kato Y, Nakajima F, Takagi M, Akita H, Masaoka T, Hattori M. 2008. Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses. Int J Syst Evol Microbiol 58: 2682-2686 doi: 10.1099/ijs.0.65543-0.

Ahetta at 23:20, 27 October 2026

2026-10-27T23:20:33Z

← Older revision		Revision as of 23:20, 27 October 2026
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	== Genome ==		== Genome ==
	[[File:Streptococcus_bovis_JB1_1_1_circos.png\|center\|thumb\|Circos plot of ''Streptococcus bovis'' JB1]]		[[File:Streptococcus_bovis_JB1_1_1_circos.png\|center\|thumb\|Circos plot of ''Streptococcus bovis'' JB1\|400x400px]]

	== DefenseFinder Results ==		== DefenseFinder Results ==

Ahetta at 23:19, 27 October 2026

2026-10-27T23:19:22Z

← Older revision		Revision as of 23:19, 27 October 2026
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	[[File:SEM_electron_micrograph_of_Streptococcus_bovis_JB1.jpg\|thumb\|300x300px\|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero]]		[[File:SEM_electron_micrograph_of_Streptococcus_bovis_JB1.jpg\|thumb\|300x300px\|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero]]

	~~[[File:S. bovis 1.jpg\|alt=kk\|thumb\|kkk]]~~

	== Introduction ==		== Introduction ==
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	== Genome ==		== Genome ==
	[[File:Streptococcus_bovis_JB1_1_1_circos.png\|center\|thumb\|Circos plot of ''~~S. azabuensis~~'' ~~KH1P5. Source: Dr. William G. Alexander~~]]		[[File:Streptococcus_bovis_JB1_1_1_circos.png\|center\|thumb\|Circos plot of ''Streptococcus bovis'' JB1]]

	== DefenseFinder Results ==		== DefenseFinder Results ==

Ahetta at 23:15, 27 October 2026

2026-10-27T23:15:38Z

← Older revision		Revision as of 23:15, 27 October 2026
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	[[File:SEM_electron_micrograph_of_Streptococcus_bovis_JB1.jpg\|thumb\|300x300px\|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero]]		[[File:SEM_electron_micrograph_of_Streptococcus_bovis_JB1.jpg\|thumb\|300x300px\|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero]]

			[[File:S. bovis 1.jpg\|alt=kk\|thumb\|kkk]]

	== Introduction ==		== Introduction ==

Ahetta: /* Culturing Conditions */

2026-10-27T23:14:42Z

Culturing Conditions

@@ Line 4: / Line 4: @@
 == Introduction ==
 ''Streptococcus bovis'' JB1 was isolated from the rumen of alfalfa-fed cow. It is a major amylolytic bacterium that depolymerize starch to glucose, fermenting it to large amounts of lactic acid that can lead to lactic acidosis in ruminants.
 == Global Methane Hub Metadata ==
@@ Line 41: / Line 42: @@
 == Culturing Conditions ==
-''Streptococcus bovis'' JB1 grows on both M2GSC and SDM (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>. Similarly, ''Streptococcus bovis'' JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>.
+''Streptococcus bovis'' JB1 grows on both M2GSC and [[SDM culture medium|SDM]] (Semi Defined medium). Cells grow overnight at 37 °C in SDM or M2GSC culture medium with head space composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>. Similarly, ''Streptococcus bovis'' JB1 grows overnight on SDM agar plates. Agar plates were placed in anaerobic Jar and incubated in anaerobic chamber under gas mixture that is composed of 95% CO<sub>2</sub> and 5% H<sub>2</sub>.
 == Antibiotic MIC Tests ==
@@ Line 72: / Line 73: @@
 == Protocols ==
-* '''Transformation protocol'''
+* '''[[SDM culture medium|Preparation of SDM culture medium]]'''
-'''Culture media:'''
+* [[Streptococcus transformation protocol|'''Transformation protocol''']]
-'''1.     For overnight culture (SGM17):'''
+== Transformation Results ==
 == External Links ==

Ahetta: /* Protocols */

2026-10-27T22:52:37Z

Protocols

@@ Line 72: / Line 72: @@
 == Protocols ==
+* '''Transformation protocol'''
 == Transformation Results ==
 == External Links ==
 [1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.