Sharpea azabuensis KH1P5 - Revision history

Mahoneykurpe: /* Genome */

2026-11-08T20:00:31Z

Genome

← Older revision		Revision as of 20:00, 8 November 2026
Line 44:		Line 44:

	== Genome ==		== Genome ==
			{\| class="wikitable"
			\|+
			! colspan="2" \|Genome characteristics
			\|-
			\|Contigs
			\|1
			\|-
			\|size (bp)
			\|2,465,508
			\|-
			\|GC %
			\|37.5
			\|-
			\|CDS
			\|2364
			\|-
			\|rRNA
			\|16
			\|-
			\|tRNA
			\|66
			\|-
			\|CRISPR
			\|2
			\|}
	[[File:S azabuensis circos.png\|Circos plot of ''S. azabuensis'' KH1P5. Source: Dr. William G. Alexander\|center\|thumb]]		[[File:S azabuensis circos.png\|Circos plot of ''S. azabuensis'' KH1P5. Source: Dr. William G. Alexander\|center\|thumb]]

Mahoneykurpe: /* Methylating E. coli Strains */

2026-10-26T09:42:18Z

Methylating E. coli Strains

← Older revision		Revision as of 09:42, 26 October 2026
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	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==
	Use a strain of ''E. coli'' that lacks dam, dcm, and hsdRMS, such as AG5589.		Use a strain of ''E. coli'' that lacks dam, dcm, and hsdRMS, such as AG5589.

	== Culturing Conditions ==		== Culturing Conditions ==
	''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.		''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.

	KH1P5 also grows well on M2G medium at 39 °C, with 10 ml cultures reaching stationary phase after 6-7 h at optical densities of ~5-6. Cells of KH1P5 ~~grow as~~ long ~~poorly-defined~~ chains.		KH1P5 also grows well on M2G medium at 39 °C, with 10 ml cultures reaching stationary phase after 6-7 h at optical densities of ~4-5. Cells of KH1P5 form long chains.

	[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]		[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]

Mahoneykurpe: /* Culturing Conditions */

2026-10-26T09:29:03Z

Culturing Conditions

← Older revision		Revision as of 09:29, 26 October 2026
Line 57:		Line 57:
	''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.		''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.

	KH1P5 also grows well on M2G medium, with 10 ml cultures reaching stationary phase after 6-7 h at optical densities of ~5-6. Cells of KH1P5 grow as long poorly-defined chains.		KH1P5 also grows well on M2G medium at 39 °C, with 10 ml cultures reaching stationary phase after 6-7 h at optical densities of ~5-6. Cells of KH1P5 grow as long poorly-defined chains.

	[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]		[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]

Mahoneykurpe: /* Culturing Conditions */

2026-10-26T09:01:36Z

Culturing Conditions

← Older revision		Revision as of 09:01, 26 October 2026
Line 55:		Line 55:

	== Culturing Conditions ==		== Culturing Conditions ==
	''S. azabuensis'' KH1P5 grows well in stock [[RCM]] ~~and M2G medium~~ at 37~~-39~~ °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer. Cells of KH1P5 grow as long poorly-defined chains.		''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C. However, the inclusion of agar in commercially made RCM causes the strain to produce a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance\|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols\|removal via washing]] with chelating agents and detergent before work with the culture. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.

			KH1P5 also grows well on M2G medium, with 10 ml cultures reaching stationary phase after 6-7 h at optical densities of ~5-6. Cells of KH1P5 grow as long poorly-defined chains.

	[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]		[[File:KH1P5 micrograph.jpg\|thumb\|Phase-contrast micrograph of ''S. azabuensis'' KH1P5 grown in M2G medium. Source: Dr. Sam Mahoney-Kurpe.]]

Mahoneykurpe: /* Culturing Conditions and Growth Characteristics */

2026-10-26T06:34:20Z