Selenomonas ruminantium HD4 - Revision history

Madachi: /* Global Methane Hub Metadata */

2026-10-30T18:23:50Z

Global Methane Hub Metadata

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	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	Note: Antimicrobial MIC was assessed using M2GSC liquid medium.		Note: Antimicrobial MIC was assessed using [[M2GSC Medium\|M2GSC]] liquid medium.
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Madachi: /* Culturing Conditions */

2026-10-29T22:14:39Z

Culturing Conditions

← Older revision		Revision as of 22:14, 29 October 2026
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	== Culturing Conditions ==		== Culturing Conditions ==
	''S. ruminantium'' HD4 grown well in [[M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''S. ruminantium'' HD4 on M10 agar is close to 96%.		''S. ruminantium'' HD4 grown well in [[M2GSC Medium\|M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''S. ruminantium'' HD4 on M10 agar is close to 96%.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Madachi: /* Methylome */

2026-10-29T17:17:52Z

Methylome

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	== Methylome ==		== Methylome ==
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	\|Motif		\|'''Motif'''
	\|Percent methylated		\|'''Percent methylated'''
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	\|GTC(6mA)NNNNNNNTGG		\|GTC(6mA)NNNNNNNTGG

Madachi: /* Global Methane Hub Metadata */

2026-10-29T17:16:51Z

Global Methane Hub Metadata

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 == Introduction ==
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 == Genome ==
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+[[File:8YPGLC 1 S.rum HD4 1 circos.png|center|thumb|300x300px|Circos plot of ''Selenomonas ruminantium'' HD4. Source: Makoto Adachi]]
 == DefenseFinder Results ==
 == Methylome ==
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 == Methylating ''E. coli'' ==
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 == Culturing Conditions ==
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+''S. ruminantium'' HD4 grown well in [[M2GSC]] and [[M10 Medium|M10]] medium at 37°C. Plating efficiency of ''S. ruminantium'' HD4 on M10 agar is close to 96%.
 == Antimicrobial MIC Tests ==
-add here
+Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
 == Protocols ==

Madachi: /* Global Methane Hub Metadata */

2026-10-29T01:02:00Z

Global Methane Hub Metadata

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 == Global Methane Hub Metadata ==
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 == Genome ==

Billkelleydonvis: Introduction and external links

2026-10-26T19:55:17Z

Introduction and external links

New page

== Introduction ==
'''''Pseudoselenomonas ruminantium'' HD4'''

''Pseudoselenomonas ruminantium'' HD4 was isolated from bovine rumen contents in the USA [1] and characterised along with other ''Selenomonas'' strains [2]. HD4 is an anaerobic bacterium that ferments a broad range of carbohydrates and produces primarily propionate, and acetate. Lactate is fermented [2] and HD4 can reduce nitrate, sulfate and fumarate.

The draft genome for HD4 (3,116,786 bp) was generated through the Hungate1000 project with JGI [3]. A 4.8 kb plasmid has been reported from HD4 [4] and a 4,899 bp plasmid was predicted from the draft genome sequence. Resequencing gave a genome of 3,180,636 bp consisting of a 2,987,729 bp chromosome and a 192,907 bp plasmid.

NCBI: ''Pseudoselenomonas ruminantium''.

GTDB: ''Selenomonas_A ruminantium_D''. HD4 belongs in a different species group than the type strain of ''P. ruminantium'' (GA192, DSM 2150).

Risk group: 1.

== Global Methane Hub Metadata ==
add here

== Genome ==
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== DefenseFinder Results ==
add here

== Methylome ==
add here

== Methylating ''E. coli'' ==
add here

== Culturing Conditions ==
add here

== Antimicrobial MIC Tests ==
add here

== Protocols ==
add here

== Transformation Results ==
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== External Links ==
[1] Huhtanen C N, Gall LS. 1953. Rumen organisms I. Curved rods and a related rod type. J Bacteriol 65, 548-553. doi: 10.1128/jb.65.5.548-553.1953.

[2] Bryant MP. 1956. The characteristics of strains of ''Selenomonas'' isolated from bovine rumen contents. J Bacteriol 72: 162-7. doi: 10.1128/jb.72.2.162-167.1956.

[2] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

[4] Martin SA, Dean RG. 1989. Characterization of a plasmid from the ruminal bacterium ''Selenomonas ruminantium''. Appl Environ Microbiol 55: 3035–3038. doi: 10.1128/aem.55.12.3035-3038.1989.