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SDM culture medium - Revision history
2026-11-14T21:15:30Z
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Ahetta: Created page with "* '''Preparation of SDM culture medium:''' '''Mineral Sol. 1 (1L)''' {| class="wikitable" |K<sub>2</sub>HPO<sub>4</sub> |6 g |} '''Mineral Sol. 2 (1L)''' {| class="wikitable" |NaCl |12 g |- |KH<sub>2</sub>PO<sub>4</sub> |6 g |- |CaCl<sub>2</sub>・2H<sub>2</sub>O |1.2 g |- |MgSO<sub>4</sub>・7H<sub>2</sub>O |1.2 g |} '''VFA Sol. (per liter)''' {| class="wikitable" |Acetic acid |13.7 ml |- |Propionic acid |6 ml |- |Butyric acid |3.68 ml |- |Isobut..."
2026-10-27T22:51:01Z
<p>Created page with "* '''Preparation of <a href="/index.php/SDM_culture_medium" title="SDM culture medium">SDM</a> culture medium:''' '''Mineral Sol. 1 (1L)''' {| class="wikitable" |K<sub>2</sub>HPO<sub>4</sub> |6 g |} '''Mineral Sol. 2 (1L)''' {| class="wikitable" |NaCl |12 g |- |KH<sub>2</sub>PO<sub>4</sub> |6 g |- |CaCl<sub>2</sub>・2H<sub>2</sub>O |1.2 g |- |MgSO<sub>4</sub>・7H<sub>2</sub>O |1.2 g |} '''VFA Sol. (per liter)''' {| class="wikitable" |Acetic acid |13.7 ml |- |Propionic acid |6 ml |- |Butyric acid |3.68 ml |- |Isobut..."</p>
<p><b>New page</b></p><div>* '''Preparation of [[SDM culture medium|SDM]] culture medium:'''<br />
<br />
'''Mineral Sol. 1 (1L)'''<br />
{| class="wikitable"<br />
|K<sub>2</sub>HPO<sub>4</sub><br />
|6 g<br />
|}<br />
'''Mineral Sol. 2 (1L)'''<br />
{| class="wikitable"<br />
|NaCl<br />
|12 g<br />
|-<br />
|KH<sub>2</sub>PO<sub>4</sub><br />
|6 g<br />
|-<br />
|CaCl<sub>2</sub>・2H<sub>2</sub>O<br />
|1.2 g<br />
|-<br />
|MgSO<sub>4</sub>・7H<sub>2</sub>O<br />
|1.2 g<br />
|}<br />
'''VFA Sol. (per liter)'''<br />
{| class="wikitable"<br />
|Acetic acid<br />
|13.7 ml<br />
|-<br />
|Propionic acid<br />
|6 ml<br />
|-<br />
|Butyric acid<br />
|3.68 ml<br />
|-<br />
|Isobutyric acid<br />
|1.1 ml<br />
|-<br />
|n-valeric acid<br />
|1.1 ml<br />
|-<br />
|isovaleric acids<br />
|1.1 ml<br />
|-<br />
|2-methylbutyric acid<br />
|0.94 ml<br />
|-<br />
|Phenylacetic acid (25uM)<br />
|3.4 mg<br />
|-<br />
|Phenylpropionic acid (25uM)<br />
|3.75 mg<br />
|-<br />
|0.2N NaOH<br />
|700 ml<br />
|}<br />
<nowiki>*</nowiki>Adjust pH7.5 and adjust volume to 1L<br />
<br />
'''Cysteine Sulfide Sol. (200ml)'''<br />
{| class="wikitable"<br />
|NaOH<br />
|1.25 g<br />
|-<br />
|Cysteine-HCl<br />
|5 g<br />
|-<br />
|Na<sub>2</sub>S.9H<sub>2</sub>O<br />
|5 g<br />
|-<br />
|ddH<sub>2</sub>O<br />
|200 ml<br />
|}<br />
- Dissolve NaOH in H<sub>2</sub>O, then boil under N<sub>2</sub>,<br />
<br />
- Cool to RT and add Cysteine-HCl and Na<sub>2</sub>S.9H<sub>2</sub>O<br />
<br />
'''Note:''' Cystine-HCl (0.5 g/L) and sodium sulfide (0.5 g/L) can be added directly to the medium.<br />
<br />
'''Hemin Sol. (100ml)'''<br />
{| class="wikitable"<br />
|Hemin<br />
|50 mg<br />
|-<br />
|1N NaOH<br />
|1 ml<br />
|}<br />
- Make to 100 ml with ddH<sub>2</sub>O<br />
<br />
- Autoclave<br />
<br />
'''Final mixture (1L)'''<br />
{| class="wikitable"<br />
|Mineral Sol. 1<br />
|50 ml<br />
|-<br />
|Mineral Sol. 2<br />
|50 ml<br />
|-<br />
|Resazurin (0.1%)<br />
|1 ml<br />
|-<br />
|Glucose<br />
|4 g<br />
|-<br />
|(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> for N source<br />
|4 g<br />
|-<br />
|Hemin Sol. (0.1%)<br />
|0.5 ml<br />
|-<br />
|Trypticase<br />
|2 g<br />
|-<br />
|Yeast extract <br />
|0.5 g<br />
|}<br />
Boiling and cool down with CO<sub>2</sub> gas<br />
<br />
'''Then add inside the anaerobic chamber'''<br />
{| class="wikitable"<br />
|VFA sol.<br />
|10 ml<br />
|-<br />
|NaHCO3<br />
|4 g<br />
|-<br />
|Cysteine sulfide Sol.<br />
|20 ml<br />
|}<br />
- Adjust pH 6.7 to 6.8 inside the chamber<br />
<br />
- Move bottle into anaerobic chamber, aliquoting medium<br />
<br />
- Autoclave</div>
Ahetta