Ruminococcus flavefaciens AE3010 - Revision history

Madachi: /* Global Methane Hub Metadata */

2026-10-30T18:18:47Z

Global Methane Hub Metadata

← Older revision		Revision as of 18:18, 30 October 2026
Line 21:		Line 21:
	\|+		\|+
	\|Origin:		\|Origin:
	\|United States of America, ~~Macike~~ lab		\|United States of America, Mackie lab
	\|-		\|-
	\|Sequenced by:		\|Sequenced by:
Line 72:		Line 72:

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	Note: Antimicrobial MIC was assessed using M2GSC liquid medium.		Note: Antimicrobial MIC was assessed using [[M2GSC Medium\|M2GSC]] liquid medium.
	{\| class="wikitable"		{\| class="wikitable"
	\|+		\|+

Madachi: /* Culturing Conditions */

2026-10-29T22:13:56Z

Culturing Conditions

← Older revision		Revision as of 22:13, 29 October 2026
Line 69:		Line 69:

	== Culturing Conditions ==		== Culturing Conditions ==
	''R. flavefaciens'' AE3010 grown well in [[M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''R. falvefaciens'' AE3010 on M10 agar is close to 66%.		''R. flavefaciens'' AE3010 grown well in [[M2GSC Medium\|M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''R. falvefaciens'' AE3010 on M10 agar is close to 66%.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Billkelleydonvis: /* Introduction */ and external links

2026-10-29T21:17:49Z

Introduction: and external links

@@ Line 6: / Line 6: @@
 ''Ruminococcus flavefaciens'' AE3010 is an obligately anaerobic, fibrolytic bacterium isolated from the rumen of a cow in New Zealand and selected as a key reference genome for the global Hungate1000 project [1]. Exhibiting the core phenotypic traits of the species, AE3010 relies on the breakdown of complex structural polysaccharides for growth, utilizing an extensive array of highly conserved cohesin and dockerin modules to assemble its specialized cell-surface cellulosome [2].
 == Global Methane Hub Metadata ==
@@ Line 100: / Line 110: @@
 [2] Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT. 2024. A genomic analysis reveals the diversity of cellulosome displaying bacteria. Front Microbiol 15:1473396.

Madachi: Created page with "Scanning electron microscopic image of ''Ruminococcus flavefaciens'' AE3010. This photo belongs to the GMH Project and was taken by Kingsley Boateng. == Introduction == '''''Ruminococcus flavefaciens''''' '''AE3010''' ''Ruminococcus flavefaciens'' AE3010 is an obligately anaerobic, fibrolytic bacterium isolated from the rumen of a cow in New Zealand and selected as a key reference genome for the global Hungate1000 pro..."

2026-10-29T00:58:40Z

Created page with "thumb|350x350px|Scanning electron microscopic image of ''Ruminococcus flavefaciens'' AE3010. <small>This photo belongs to the GMH Project and was taken by Kingsley Boateng.</small> == Introduction == '''''Ruminococcus flavefaciens''''' '''AE3010''' ''Ruminococcus flavefaciens'' AE3010 is an obligately anaerobic, fibrolytic bacterium isolated from the rumen of a cow in New Zealand and selected as a key reference genome for the global Hungate1000 pro..."

New page

[[File:Image4.png|thumb|350x350px|Scanning electron microscopic image of ''Ruminococcus flavefaciens'' AE3010.
<small>This photo belongs to the GMH Project and was taken by Kingsley Boateng.</small>]]

== Introduction ==
'''''Ruminococcus flavefaciens''''' '''AE3010'''

''Ruminococcus flavefaciens'' AE3010 is an obligately anaerobic, fibrolytic bacterium isolated from the rumen of a cow in New Zealand and selected as a key reference genome for the global Hungate1000 project [1]. Exhibiting the core phenotypic traits of the species, AE3010 relies on the breakdown of complex structural polysaccharides for growth, utilizing an extensive array of highly conserved cohesin and dockerin modules to assemble its specialized cell-surface cellulosome [2].

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|United States of America, Macike lab
|-
|Sequenced by:
|Makoto Adachi
|-
|Assembled by:
|William G. Alexander
|-
|Methylome called by:
|William G. Alexander
|-
|Methyltransferases cloned by:
|Roy Rodriguez Carrero
|-
|MIC and Culturing Conditions by:
|Roy Rodriguez Carrero
|-
|Transformation by:
|
|}

== Genome ==
[[File:55RBRH 1 Rum E3010 1 circos.png|center|thumb|300x300px|Circos plot of ''R. flavefaciens'' AE3010. Source: Makoto Adachi]]

== DefenseFinder Results ==

== Methylome ==
{| class="wikitable"
| valign="middle" |Motif
| valign="middle" |Percent methylated
|-
| valign="middle" |TGAA(6mA)T
| valign="middle" |99.49
|-
| valign="middle" |CTTG(6mA)YG
| valign="middle" |99.76
|-
| valign="middle" |CG(6mA)CC
| valign="middle" |97.15
|-
| valign="middle" |CACA(6mA)T
| valign="middle" |97.75
|}

== Methylating ''E. coli'' ==
add here

== Culturing Conditions ==
''R. flavefaciens'' AE3010 grown well in [[M2GSC]] and [[M10 Medium|M10]] medium at 37°C. Plating efficiency of ''R. falvefaciens'' AE3010 on M10 agar is close to 66%.

== Antimicrobial MIC Tests ==
Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
{| class="wikitable"
|+
!'''Antibiotic'''
!'''Concentration (µg/ml)'''
|-
|Ampicilin
|0.5
|-
|Chlorhamphenicol
|0.3
|-
|Erythromycin
|1.6
|-
|Gentamycin
|0.5
|-
|Kanamycin
|4.7
|-
|Streptomycin
|0.5
|-
|Tetracycline
|0.1
|}

== Protocols ==
add here

== Transformation Results ==
add here

== External Links ==
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D, Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nat Biotechnol 36:359–367.

[2] Minor CM, Takayesu A, Ha SM, Salwinski L, Sawaya MR, Pellegrini M, Clubb RT. 2024. A genomic analysis reveals the diversity of cellulosome displaying bacteria. Front Microbiol 15:1473396.