Ruminococcus albus 8 - Revision history

Madachi: /* Global Methane Hub Metadata */

2026-10-30T18:17:03Z

Global Methane Hub Metadata

← Older revision		Revision as of 18:17, 30 October 2026
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	\|+		\|+
	\|Origin:		\|Origin:
	\|United States of America, ~~Macike~~ lab		\|United States of America, Mackie lab
	\|-		\|-
	\|Sequenced by:		\|Sequenced by:
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	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	Note: Antimicrobial MIC was assessed using M2GSC liquid medium.		Note: Antimicrobial MIC was assessed using [[M2GSC Medium\|M2GSC]] liquid medium.
	{\| class="wikitable"		{\| class="wikitable"
	\|+		\|+

Madachi: /* Culturing Conditions */

2026-10-29T22:11:25Z

Culturing Conditions

← Older revision		Revision as of 22:11, 29 October 2026
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	== Culturing Conditions ==		== Culturing Conditions ==
	''R. albus'' 8 grown well in [[M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''R. albus'' 8 on M10 agar is close to 89%.		''R. albus'' 8 grown well in [[M2GSC Medium\|M2GSC]] and [[M10 Medium\|M10]] medium at 37°C. Plating efficiency of ''R. albus'' 8 on M10 agar is close to 89%.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Madachi: /* Introduction */

2026-10-29T21:29:40Z

Introduction

← Older revision		Revision as of 21:29, 29 October 2026
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	While ''R. albus'' 8 shares core phenotypic characteristics with other strains, genomic and transcriptomic analyses indicate that it exhibits a specialized ecological preference for hemicellulose and xylan degradation over highly crystalline cellulose, distinguishing it from the highly cellulolytic ''R. albus'' 7 [1]. The strain secretes multiple endoxylanases and utilizes a unique phosphoketolase pathway for the rapid catabolism of pentose sugars, producing acetate, ethanol, formate, and hydrogen [2]. In the rumen, ''R. albus'' 8 contributes to the energy acquisition of the host animal through the production of volatile fatty acids, while acting in syntrophy with methanogenic archaea by providing the hydrogen necessary for enteric methane production.		While ''R. albus'' 8 shares core phenotypic characteristics with other strains, genomic and transcriptomic analyses indicate that it exhibits a specialized ecological preference for hemicellulose and xylan degradation over highly crystalline cellulose, distinguishing it from the highly cellulolytic ''R. albus'' 7 [1]. The strain secretes multiple endoxylanases and utilizes a unique phosphoketolase pathway for the rapid catabolism of pentose sugars, producing acetate, ethanol, formate, and hydrogen [2]. In the rumen, ''R. albus'' 8 contributes to the energy acquisition of the host animal through the production of volatile fatty acids, while acting in syntrophy with methanogenic archaea by providing the hydrogen necessary for enteric methane production.

			LPSN: ''Ruminococcus albus'' Hungate 1957

			NCBI: ''Ruminococcus albus'' 8

			GTDB: ''Ruminococcus albus''

			Risk group: 1

	== Global Methane Hub Metadata ==		== Global Methane Hub Metadata ==

Madachi: /* Methylome */

2026-10-28T23:52:57Z

Methylome

← Older revision		Revision as of 23:52, 28 October 2026
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	== Methylome ==		== Methylome ==
	{\| class="wikitable"		{\| class="wikitable"
	\|+
	!Motif		!Motif
	!Percent methylated		!Percent methylated
	\|-		\|-
	\|		\|G(6mA)TCC
	\|		\|99.53
	\|-		\|-
	\|		\|GG(6mA)TC
	\|		\|99.93
	\|-		\|-
	\|		\|GG(6mA)GG
	\|		\|98.88
	\|-		\|-
	\|		\|GNC(6mA)YNNNNNNCCT
	\|		\|97.47
	\|-		\|-
	\|		\|(6mA)GGNNNNNNRTGNC
	\|		\|99.28
	\|-		\|-
	\|		\|G(6mA)TNNNNCTCA
	\|		\|98.36
	\|-		\|-
	\|		\|TG(6mA)GNNNNATC
	\|		\|97.64
	\|-		\|-
	\|		\|A(5mC)GGG
	\|		\|97.16
	\|-		\|-
	\|		\|C(5mC)CGT
	\|		\|87.25
	\|-		\|-
	\|		\|G(5mC)CTCC
	\|		\|85.17
	\|}		\|}

Madachi: /* Antimicrobial MIC Tests */

2026-10-28T23:48:53Z

Antimicrobial MIC Tests

← Older revision		Revision as of 23:48, 28 October 2026
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	\|Streptomycin		\|Streptomycin
	\|Resistance (>200)		\|Resistance (>200)
			\|-
			\|Tetracycline
			\|1
	\|}		\|}

Madachi at 23:43, 28 October 2026

2026-10-28T23:43:56Z

@@ Line 1: / Line 1: @@
 == Introduction ==
-add here
+'''''Ruminococcus albus''''' '''8'''
 == Global Methane Hub Metadata ==
@@ Line 19: / Line 25: @@
 |-
 |Methyltransferases cloned by:
+|Roy Rodriguez Carrero
 |-
 |MIC and Culturing Conditions by:
+|Roy Rodriguez Carrero
 |-
 |Transformation by:
@@ Line 29: / Line 35: @@
 == Genome ==
-add here
+[[File:2N52CS 1 R8 1 circos.png|center|thumb|300x300px|Circos plot of ''R. albus'' 8. Source: Makoto Adachi]]
 == DefenseFinder Results ==
 == Methylome ==
-add here
+{| class="wikitable"
 == Methylating ''E. coli'' ==
@@ Line 41: / Line 80: @@
 == Culturing Conditions ==
-add here
+''R. albus'' 8 grown well in [[M2GSC]] and [[M10 Medium|M10]] medium at 37°C. Plating efficiency of ''R. albus'' 8 on M10 agar is close to 89%.
 == Antimicrobial MIC Tests ==
-add here
+Note: Antimicrobial MIC was assessed using M2GSC liquid medium.
 == Protocols ==
@@ Line 53: / Line 115: @@
 == External Links ==
-add here
+[1] Kwon I. 2016. Comparative whole genome transcriptional responses of ''Ruminococcus albus'' strain 7 and 8: a case for specialization and niche differentiation. Ph.D. dissertation. University of Illinois at Urbana-Champaign, Urbana, IL.

Madachi: Added the Grobal Methane Hub Metadata

2026-10-16T00:18:41Z

Added the Grobal Methane Hub Metadata

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 == Global Methane Hub Metadata ==
-add here
+{| class="wikitable"
 == Genome ==

Madachi: Pased the strain template

2026-10-15T23:37:57Z

Pased the strain template

New page

== Introduction ==
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== Global Methane Hub Metadata ==
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== Genome ==
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== DefenseFinder Results ==
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== Methylome ==
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== Methylating ''E. coli'' ==
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== Culturing Conditions ==
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== Antimicrobial MIC Tests ==
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== Protocols ==
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== Transformation Results ==
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== External Links ==
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