Prevotella ruminicola 23T - Revision history

Yuqili at 18:02, 30 October 2026

2026-10-30T18:02:06Z

← Older revision		Revision as of 18:02, 30 October 2026
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	== Culturing Conditions ==		== Culturing Conditions ==
	''Prevotella ruminicola'' 23 grows well in M2GSC or M2G medium at 37 °C under strictly anaerobic conditions. ''P. ruminicola'' 23 can also be cultivated on M10G agar plates. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.		''Prevotella ruminicola'' 23 grows well in [[M2GSC Medium\|M2GSC]] or M2G medium at 37 °C under strictly anaerobic conditions. ''P. ruminicola'' 23 can also be cultivated on M10G agar plates. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Yuqili at 22:48, 28 October 2026

2026-10-28T22:48:26Z

← Older revision		Revision as of 22:48, 28 October 2026
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			[[File:SEMP23.jpg\|thumb\|Scanning electron microscopy image of ''Prevotella ruminicola'' 23. Source: Yuqi Li]]

	== Introduction ==		== Introduction ==
	'''''Prevotella ruminicola'' 23<sup>T</sup>''' (ATCC 19189, DSM 19721), also known as ''Xylanibacter ruminicola'' 23, is an obligately anaerobic, Gram-negative bacterium originally isolated from the bovine rumen [1]. It is the type strain of ''P. ruminicola'' and was originally described as ''Bacteroides ruminicola'' by Bryant et al. in 1958 [1]. The species was subsequently transferred to the genus ''Prevotella'' in 1990 [2].		'''''Prevotella ruminicola'' 23<sup>T</sup>''' (ATCC 19189, DSM 19721), also known as ''Xylanibacter ruminicola'' 23, is an obligately anaerobic, Gram-negative bacterium originally isolated from the bovine rumen [1]. It is the type strain of ''P. ruminicola'' and was originally described as ''Bacteroides ruminicola'' by Bryant et al. in 1958 [1]. The species was subsequently transferred to the genus ''Prevotella'' in 1990 [2].

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T22:42:42Z

Antimicrobial MIC Tests

← Older revision		Revision as of 22:42, 28 October 2026
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	\|-		\|-
	\|Erythromycin		\|Erythromycin
	\|1.3		\|1.29
	\|}		\|}

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T22:41:48Z

Antimicrobial MIC Tests

← Older revision		Revision as of 22:41, 28 October 2026
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	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
			[[File:P23MIC.jpg\|left\|thumb\|MIC tests of ''P. ruminicola'' 23. Source: Yuqi Li]]
			{\| class="wikitable"
			!Antibiotic
			!Concentration ('''μ'''g/mL)
			\|-
			\|Ampicillin
			\|Resistant (>200 μg/mL)
			\|-
			\|Kanamycin
			\|Resistant (>200 μg/mL)
			\|-
			\|Chloramphenicol
			\|1.03
			\|-
			\|Tetracycline
			\|0.31
			\|-
			\|Gentamicin
			\|Resistant (>200 μg/mL)
			\|-
			\|Streptomycin
			\| < 0.0625
			\|-
			\|Erythromycin
			\|1.3
			\|}

	== External Links ==		== External Links ==

Yuqili: /* Global Methane Hub Metadata */

2026-10-28T22:32:19Z

Global Methane Hub Metadata

← Older revision		Revision as of 22:32, 28 October 2026
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	== Culturing Conditions ==		== Culturing Conditions ==
			''Prevotella ruminicola'' 23 grows well in M2GSC or M2G medium at 37 °C under strictly anaerobic conditions. ''P. ruminicola'' 23 can also be cultivated on M10G agar plates. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Yuqili: /* Global Methane Hub Metadata */

2026-10-28T22:28:49Z

Global Methane Hub Metadata

← Older revision		Revision as of 22:28, 28 October 2026
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	== Global Methane Hub Metadata ==		== Global Methane Hub Metadata ==
			{\| class="wikitable"
			\|Origin:
			\|Illinois, Mackie Lab
			\|-
			\|Sequenced by:
			\|Yuqi Li
			\|-
			\|Assembled by:
			\|William G. Alexander
			\|-
			\|Methylome called by:
			\|William G. Alexander
			\|-
			\|MIC and Culturing Conditions by:
			\|Yuqi Li
			\|}

	== Genome ==		== Genome ==

Yuqili: /* Introduction */

2026-10-28T21:15:33Z

Introduction

← Older revision		Revision as of 21:15, 28 October 2026
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	== Introduction ==		== Introduction ==
	''Prevotella ruminicola'' 23 (ATCC 19189, DSM 19721), also known as ''Xylanibacter ruminicola'' 23, is an obligately anaerobic, Gram-negative bacterium originally isolated from the bovine rumen [1]. It is the type strain of ''P. ruminicola'' and was originally described as ''Bacteroides ruminicola'' by Bryant et al. in 1958 [1]. The species was subsequently transferred to the genus ''Prevotella'' in 1990 [2].		'''''Prevotella ruminicola'' 23<sup>T</sup>''' (ATCC 19189, DSM 19721), also known as ''Xylanibacter ruminicola'' 23, is an obligately anaerobic, Gram-negative bacterium originally isolated from the bovine rumen [1]. It is the type strain of ''P. ruminicola'' and was originally described as ''Bacteroides ruminicola'' by Bryant et al. in 1958 [1]. The species was subsequently transferred to the genus ''Prevotella'' in 1990 [2].

	''P. ruminicola'' 23 contributes to the degradation of plant polysaccharides in the rumen, particularly xylan and other hemicelluloses. Its genome encodes numerous carbohydrate-active enzymes involved in the degradation of non-cellulosic polysaccharides [3]. Several xylan-degrading enzymes have been characterized, including a bifunctional xylanase/endoglucanase and a GH10 xylanase–ferulic acid esterase [4, 5].		''P. ruminicola'' 23 contributes to the degradation of plant polysaccharides in the rumen, particularly xylan and other hemicelluloses. Its genome encodes numerous carbohydrate-active enzymes involved in the degradation of non-cellulosic polysaccharides [3]. Several xylan-degrading enzymes have been characterized, including a bifunctional xylanase/endoglucanase and a GH10 xylanase–ferulic acid esterase [4, 5].

Yuqili: /* Genome */

2026-10-28T21:14:45Z

Genome

← Older revision		Revision as of 21:14, 28 October 2026
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	== Genome ==		== Genome ==
	[[File:Circos plot of P. ruminicola 23.png~~\|none~~\|thumb\|Circos plot of ''P. ruminicola'' 23<sup>T</sup>. Source: Yuqi Li]]		[[File:Circos plot of P. ruminicola 23.png\|thumb\|Circos plot of ''P. ruminicola'' 23<sup>T</sup>. Source: Yuqi Li\|center]]

	== Methylome ==		== Methylome ==

Yuqili at 21:14, 28 October 2026

2026-10-28T21:14:17Z

← Older revision		Revision as of 21:14, 28 October 2026
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	== Genome ==		== Genome ==
			[[File:Circos plot of P. ruminicola 23.png\|none\|thumb\|Circos plot of ''P. ruminicola'' 23<sup>T</sup>. Source: Yuqi Li]]

	== Methylome ==		== Methylome ==

Yuqili at 21:03, 28 October 2026

2026-10-28T21:03:57Z

← Older revision		Revision as of 21:03, 28 October 2026
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	\|77.28		\|77.28
	\|}		\|}

			== Culturing Conditions ==

			== Antimicrobial MIC Tests ==

	== External Links ==		== External Links ==