Prevotella bryantii B14 - Revision history

Yuqili at 17:18, 30 October 2026

2026-10-30T17:18:30Z

← Older revision		Revision as of 17:18, 30 October 2026
Line 74:		Line 74:

	== Culturing Conditions ==		== Culturing Conditions ==
	''Prevotella bryantii'' B<sub>1</sub>4 grows well in M2GSC or M2G medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in either medium. B<sub>1</sub>4 can also be grown on M10G agar plates, with colonies typically appearing after 36–72 h of incubation. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.		''Prevotella bryantii'' B<sub>1</sub>4 grows well in [[M2GSC Medium\|M2GSC]] or M2G medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in either medium. B<sub>1</sub>4 can also be grown on M10G agar plates, with colonies typically appearing after 36–72 h of incubation. Cultures and agar plates are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==

Yuqili at 17:17, 30 October 2026

2026-10-30T17:17:49Z

← Older revision		Revision as of 17:17, 30 October 2026
Line 1:		Line 1:
	[[File:B14SEM.jpg\|thumb\|Scanning electron microscopy ~~(SEM)~~ image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology. Source: Yuqi Li]]		[[File:B14SEM.jpg\|thumb\|Scanning electron microscopy image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology. Source: Yuqi Li]]

	== Introduction ==		== Introduction ==

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T22:51:29Z

Antimicrobial MIC Tests

← Older revision		Revision as of 22:51, 28 October 2026
Line 77:		Line 77:

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	[[File:Data 2.jpg~~\|none~~\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4. Source: Yuqi Li]]		[[File:Data 2.jpg\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4. Source: Yuqi Li\|left]]
	{\| class="wikitable"		{\| class="wikitable"
	!Antibiotic		!Antibiotic

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T22:40:22Z

Antimicrobial MIC Tests

← Older revision		Revision as of 22:40, 28 October 2026
Line 98:		Line 98:
	\|-		\|-
	\|Streptomycin		\|Streptomycin
	\|Resistant (>200 ug/mL)		\|Resistant (>200 μg/mL)
	\|-		\|-
	\|Erythromycin		\|Erythromycin

Yuqili: /* Introduction */

2026-10-28T22:26:27Z

Introduction

← Older revision		Revision as of 22:26, 28 October 2026
Line 4:		Line 4:
	'''''Segatella bryantii'' B<sub>1</sub>4'''		'''''Segatella bryantii'' B<sub>1</sub>4'''

	''Segatella bryantii'' B<sub>1</sub>4 (DSM 11371), formerly ''Prevotella bryantii'', was isolated from rumen contents of a Holstein cow fed alfalfa hay in the USA [1]. It was originally named as ''Bacteroides ruminicola'' subsp. ''brevis'' and subsequently changed to ''Prevotella bryantii'' [2]. The strain was originally designated B<sub>1</sub>4 but ~~B14~~ is commonly used. ~~B14~~ has been the focus of many studies and is able to ferment a wide range of carbohydrates [3, 4]. The genes involved in xylan degradation have been characterized [5].		''Segatella bryantii'' B<sub>1</sub>4 (DSM 11371), formerly ''Prevotella bryantii'', was isolated from rumen contents of a Holstein cow fed alfalfa hay in the USA [1]. It was originally named as ''Bacteroides ruminicola'' subsp. ''brevis'' and subsequently changed to ''Prevotella bryantii'' [2]. The strain was originally designated B<sub>1</sub>4 but B<sub>1</sub>4 is commonly used. B<sub>1</sub>4 has been the focus of many studies and is able to ferment a wide range of carbohydrates [3, 4]. The genes involved in xylan degradation have been characterized [5].

	Taxonomic analysis of the genus ''Prevotella'' lists B14 as the ''Segatella bryantii'' type strain [6]. However, NCBI now records B14 and C21a as belonging to the species ''Segatella baroniae'', although the reason for this is unclear.		Taxonomic analysis of the genus ''Prevotella'' lists B14 as the ''Segatella bryantii'' type strain [6]. However, NCBI now records B14 and C21a as belonging to the species ''Segatella baroniae'', although the reason for this is unclear.

Yuqili at 19:15, 28 October 2026

2026-10-28T19:15:46Z

← Older revision		Revision as of 19:15, 28 October 2026
Line 1:		Line 1:
	[[File:B14SEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology.]]		[[File:B14SEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology. Source: Yuqi Li]]

	== Introduction ==		== Introduction ==

Yuqili at 19:15, 28 October 2026

2026-10-28T19:15:13Z

← Older revision		Revision as of 19:15, 28 October 2026
Line 1:		Line 1:
			[[File:B14SEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Prevotella bryantii'' B<sub>1</sub>4, showing its characteristic rod-shaped morphology.]]

	== Introduction ==		== Introduction ==
	'''''Segatella bryantii'' B<sub>1</sub>4'''		'''''Segatella bryantii'' B<sub>1</sub>4'''

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T19:06:51Z

Antimicrobial MIC Tests

@@ Line 75: / Line 75: @@
 == Antimicrobial MIC Tests ==
-[[File:Data 2.jpg|none|thumb|MIC tests of ''P. bryantii'' B<sub>1</sub>4]]
+[[File:Data 2.jpg|none|thumb|MIC tests of ''P. bryantii'' B<sub>1</sub>4. Source: Yuqi Li]]
 == Protocols ==

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T19:01:38Z

Antimicrobial MIC Tests

← Older revision		Revision as of 19:01, 28 October 2026
Line 75:		Line 75:

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	[[File:Data 1.jpg\|none\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4]]		[[File:Data 2.jpg\|none\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4]]

	== Protocols ==		== Protocols ==

Yuqili: /* Antimicrobial MIC Tests */

2026-10-28T19:00:20Z

Antimicrobial MIC Tests

← Older revision		Revision as of 19:00, 28 October 2026
Line 75:		Line 75:

	== Antimicrobial MIC Tests ==		== Antimicrobial MIC Tests ==
	[[File:~~MICB14~~.~~png~~\|none\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4]]		[[File:Data 1.jpg\|none\|thumb\|MIC tests of ''P. bryantii'' B<sub>1</sub>4]]

	== Protocols ==		== Protocols ==