Billkelleydonvis: Introduction and external links

2026-10-28T19:48:29Z

Introduction and external links

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== Introduction ==
'''''Olsenella'' sp. KHGC19'''

''Olsenella'' sp. KHGC19 (DSM 115844) was isolated from bovine rumen contents in NZ using KH medium which was supplemented with haemin and vitamin K [1]. KHGC19 is a non-motile, non-spore forming Gram positive, anaerobe which produces D-lactate as the main product from glucose fermentation. KHGC19 is a high G+C lactic acid bacterium and while originally identified as ''Olsenella'' it was subsequently assigned to the genus ''Parafannyhessea'' within the family ''Atopobiaceae'' [2]. However, its smaller genome size and repertoire of carbohydrate active enzymes is distinct from that for the type strain of ''Parafannyhessea umbonata'' (lac31, DSM 22620).

KHGC19 is of interest as it is one of the few rumen bacteria whose genome encodes the gene cluster for the enzyme choline trimethylamine lyase (CTMAL; EC:4.3.99.4) which generates trimethylamine a substrate for methylotrophic methanogens, especially members of the ''Methanomassiliicoccales'' [3]. In KHGC19, CTMAL is an enzyme complex composed of a catalytic choline utilisation polypeptide CutC, and an associated activating protein CutD, encoded by a 21 gene cluster that also contains genes encoding bacterial microcompartment proteins. A similar, slightly larger, CTMAL gene cluster is found in the type strain of ''Parafannyhessea umbonata''.

The draft genome for KHGC19 (2,057,956 bp) was generated through the Hungate1000 project with JGI [1].

NCBI: ''Parafannyhessea umbonata.''

GTDB: ''Olsenella_J umbonata_A.''

Risk group: 1.

== Global Methane Hub Metadata ==
{| class="wikitable"
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|Origin:
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|Sequenced by:
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|Assembled by:
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|Methylome called by:
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|Methyltransferases cloned by:
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|MIC and Culturing Conditions by:
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|Transformation by:
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== Genome ==

== DefenseFinder Results ==

== Methylome ==

== Methylating E. coli Strains ==

== Culturing Conditions ==

== Antibiotic MIC Tests ==

== Protocols ==

== Transformation Results ==

== External Links ==
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

[2] Zgheib R, Anani H, Meng MM, Mailhe M, Ricaboni D, Morand A, Caputo A, Traore SI, Fontanini A, Armstrong N, Raoult D, Fournier PE, 2021. New human-associated species of the family ''Atopobiaceae'' and proposal to reclassify members of the genus ''Olsenella''. Int J Syst Evol Microbiol 71. doi: 10.1099/ijsem.0.004819.

[3] Kelly WJ, Leahy SC, Kamke J, Soni P, Koike S, Mackie R, Seshadri R, Cook GM, Morales SE, Greening C, Attwood GT. 2019. Occurrence and expression of genes encoding methyl-compound production in rumen bacteria. Anim Microbiome 1: 15. doi: 10.1186/s42523-019-0016-0.

Olsenella sp. KHGC19 - Revision history

Billkelleydonvis: Introduction and external links