Methanobrevibacter olleyae 1H5-1PT - Revision history

Sharik at 18:46, 28 October 2026

2026-10-28T18:46:49Z

← Older revision		Revision as of 18:46, 28 October 2026
Line 29:		Line 29:

	== Genome ==		== Genome ==
			Single contig of 2,173,952 bp.

	== DefenseFinder Results ==		== DefenseFinder Results ==
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	== Culturing Conditions ==		== Culturing Conditions ==
	''M. olleyae'' 1H5-1PT grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe ~~incubators~~ filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S .		''M. olleyae'' 1H5-1PT is a strict anaerobe and grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S .

	== MIC Tests ==		== MIC Tests ==

Sharik: conditions

2026-10-28T18:13:45Z

conditions

← Older revision		Revision as of 18:13, 28 October 2026
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	== Methylome ==		== Methylome ==
	No methylation motifs were detected in ''~~Streptococcus bovis~~'' ~~JB1~~.		No methylation motifs were detected in ''Methanobrevibacter olleyae'' 1H5-1PT.

	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==
	No ''E. coli'' methylation strain has been constructed for ''~~S. bovis~~'' ~~JB1 strain~~		No ''E. coli'' methylation strain has been constructed for ''Methanobrevibacter olleyae'' 1H5-1PT.

	== Culturing Conditions ==		== Culturing Conditions ==
			''M. olleyae'' 1H5-1PT grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubators filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S .

	== ~~Antibiotic~~ MIC Tests ==		== MIC Tests ==
	{\| class="wikitable"		{\| class="wikitable"
	!antibiotic		!antibiotic
	!concentration (ug/mL)		!concentration (ug/mL)
	\|-		\|-
	\|~~Ampicillin~~		\|Puromycin
	\|5		\|
	\|-		\|-
	\|~~Kanamycin~~		\|Nourseothricin
	\|~~0.38~~		\|
	\|-		\|-
	\|~~Chloramphenicol~~		\|Neomycin
	\|25		\|
	\|-		\|-
	\|~~Tetracycline~~		\|8-ADP
	\|1		\|
	\|-		\|-
	\|~~Gentamicin~~		\|5-Fluorouracil
	\|~~insensitive~~		\|
	\|-		\|-
	\|~~Streptomycin~~		\|2-BES
	\|96		\|
	\|-		\|-
	\|~~Erythromycin~~		\|5-FOA
	\|~~0.23~~		\|
	\|}		\|}

Sharik: /* Global Methane Hub Metadata */

2026-10-28T17:18:28Z

Global Methane Hub Metadata

← Older revision		Revision as of 17:18, 28 October 2026
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	\|Transformation by:		\|Transformation by:
	\|Sharik Khan, University of Illinois at Urbana-Champaign		\|Sharik Khan, University of Illinois at Urbana-Champaign
	Komal ~~Aggrawal~~, University of Illinois at Urbana-Champaign		Komal Agrawal, University of Illinois at Urbana-Champaign
	\|}		\|}

Sharik: metadata

2026-10-28T17:16:16Z

metadata

New page

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexande, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|N/A
|-
|MIC and Culturing Conditions by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|Sharik Khan, University of Illinois at Urbana-Champaign
Komal Aggrawal, University of Illinois at Urbana-Champaign
|}

== Genome ==

== DefenseFinder Results ==

== Methylome ==
No methylation motifs were detected in ''Streptococcus bovis'' JB1.

== Methylating E. coli Strains ==
No ''E. coli'' methylation strain has been constructed for ''S. bovis'' JB1 strain

== Culturing Conditions ==

== Antibiotic MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Ampicillin
|5
|-
|Kanamycin
|0.38
|-
|Chloramphenicol
|25
|-
|Tetracycline
|1
|-
|Gentamicin
|insensitive
|-
|Streptomycin
|96
|-
|Erythromycin
|0.23
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==