Sharik: /* Antibiotic MIC Tests */

2026-10-28T18:46:41Z

Antibiotic MIC Tests

← Older revision		Revision as of 18:46, 28 October 2026
Line 28:		Line 28:

	== Genome ==		== Genome ==
			Single contig of 2,084,047 bp.

	== DefenseFinder Results ==		== DefenseFinder Results ==

	== Methylome ==		== Methylome ==
	~~No methylation~~ motifs ~~were detected in~~ ''~~Streptococcus bovis~~'' ~~JB1~~.		{\| class="wikitable"
			\| valign="top" \|'''#6mA modified motifs'''
			\| valign="top" \|
			\| valign="top" \|
			\| valign="top" \|
			\|-
			\| valign="top" \|'''#motif'''
			\| valign="top" \|methylCounts
			\| valign="top" \|totalCounts
			\| valign="top" \|%motifsMethyl
			\|-
			\| valign="top" \|'''C(6mA)TCC'''
			\| valign="top" \|2452
			\| valign="top" \|2922
			\| valign="top" \|83.92
			\|-
			\| valign="top" \|'''GATG(6mA)T'''
			\| valign="top" \|2170
			\| valign="top" \|2556
			\| valign="top" \|84.90
			\|-
			\| valign="top" \|'''GG(6mA)TG'''
			\| valign="top" \|2471
			\| valign="top" \|2922
			\| valign="top" \|84.57
			\|-
			\| valign="top" \|'''(6mA)GGNNNNNNRTC'''
			\| valign="top" \|1037
			\| valign="top" \|1342
			\| valign="top" \|77.27
			\|-
			\| valign="top" \|'''G(6mA)YNNNNNNCCT'''
			\| valign="top" \|984
			\| valign="top" \|1342
			\| valign="top" \|73.32
			\|}

	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==
	~~No ''E. coli'' methylation strain has been constructed for ''S. bovis'' JB1 strain~~

	== Culturing Conditions ==		== Culturing Conditions ==
			''Methanobrevibacter boviskoreani'' JH1T is a strict anaerobe and can be grown in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v). Growth of ''M. boviskoreani JH1T'' requires rumen fluid but does not need volatile fatty acids (VFAs). Upon plating, the strain forms colonies in 7-10 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H<sub>2</sub>/CO<sub>2</sub> (80:20) supplemented with 1000ppm H<sub>2</sub>S .

	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==
Line 44:		Line 80:
	!concentration (ug/mL)		!concentration (ug/mL)
	\|-		\|-
	\|~~Ampicillin~~		\|Puromycin
	\|5		\|
	\|-		\|-
	\|~~Kanamycin~~		\|Nourseothricin
	\|~~0.38~~		\|
	\|-		\|-
	\|~~Chloramphenicol~~		\|Neomycin
	\|25		\|
	\|-		\|-
	\|~~Tetracycline~~		\|8-ADP
	\|1		\|
	\|-		\|-
	\|~~Gentamicin~~		\|5-Fluorouracil
	\|~~insensitive~~		\|
	\|-		\|-
	\|~~Streptomycin~~		\|2-BES
	\|96		\|
	\|-		\|-
	\|~~Erythromycin~~		\|5-FOA
	\|~~0.23~~		\|
	\|}		\|}

Sharik: metadata

2026-10-28T17:29:04Z

metadata

New page

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexande, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|Adam Guss, Oak Ridge National Laboratory
|-
|MIC and Culturing Conditions by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|
|}

== Genome ==

== DefenseFinder Results ==

== Methylome ==
No methylation motifs were detected in ''Streptococcus bovis'' JB1.

== Methylating E. coli Strains ==
No ''E. coli'' methylation strain has been constructed for ''S. bovis'' JB1 strain

== Culturing Conditions ==

== Antibiotic MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Ampicillin
|5
|-
|Kanamycin
|0.38
|-
|Chloramphenicol
|25
|-
|Tetracycline
|1
|-
|Gentamicin
|insensitive
|-
|Streptomycin
|96
|-
|Erythromycin
|0.23
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter boviskoreani JH1T - Revision history

Sharik: /* Antibiotic MIC Tests */

Sharik: metadata