MMSM - Revision history

https://gmhwiki.alexandersci.com/index.php?action=history&feed=atom&title=MMSM MMSM - Revision history 2026-11-14T21:10:28Z Revision history for this page on the wiki MediaWiki 1.46.2 https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=941&oldid=prev Nanashao: Blanked the page 2026-10-16T18:19:14Z

<p>Blanked the page</p> <a href="https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=941&oldid=938">Show changes</a>

Nanashao https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=938&oldid=prev Nanashao: /* Sources */ 2026-10-16T15:22:15Z

<p><span class="autocomment">Sources</span></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:22, 16 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l226">Line 226:</td> <td colspan="2" class="diff-lineno">Line 226:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Sources ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Sources ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>* <del style="font-weight: bold; text-decoration: none;">[https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] </del>(Used by Metcalf and Whitman Lab)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>* <ins style="font-weight: bold; text-decoration: none;">Reference </ins>(Used by Metcalf and Whitman Lab)</div></td></tr>  </table>

Nanashao https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=937&oldid=prev Nanashao: /* Sources */ 2026-10-16T15:20:50Z

<p><span class="autocomment">Sources</span></p> <table style="background-color: #fff; color: #202122;" data-mw-interface=""> <col class="diff-marker" /> <col class="diff-content" /> <col class="diff-marker" /> <col class="diff-content" /> <tr class="diff-title" lang="en"> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">← Older revision</td> <td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 15:20, 16 October 2026</td> </tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l226">Line 226:</td> <td colspan="2" class="diff-lineno">Line 226:</td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Sources ==</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>== Sources ==</div></td></tr> <tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><br></td></tr> <tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>* [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by <del style="font-weight: bold; text-decoration: none;">Guss </del>Lab)</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>* [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by <ins style="font-weight: bold; text-decoration: none;">Metcalf and Whitman </ins>Lab)</div></td></tr>  </table>

Nanashao https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=936&oldid=prev Nanashao at 15:19, 16 October 2026 2026-10-16T15:19:34Z

<p></p> <a href="https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=936&oldid=934">Show changes</a>

Nanashao https://gmhwiki.alexandersci.com/index.php?title=MMSM&diff=934&oldid=prev Nanashao: Created page with " == Description == Modified ''Methanobrevibacter smithii'' and ''Methanospheara stadtmanae'' medium (MMSM) is a rich, undefined medium commonly used for the anaerobic cultivation of ''Methanobrevibacter'' species. == Recipe == {| class="wikitable" |'''Composition per 100 mL''' |'''Amount''' |- |''Modified Rich medium for Methanobrevibacter (MMSM)'' | |- |Mineral 1 |3.3 mL |- |Mineral 2 |3.3 mL |- |Yeast extract |0.2 g |- |Tryptone |0.2 g |- |Na Acetate·3H<sub>2</s..." 2026-10-16T15:13:51Z

<p>Created page with " == Description == Modified ''Methanobrevibacter smithii'' and ''Methanospheara stadtmanae'' medium (MMSM) is a rich, undefined medium commonly used for the anaerobic cultivation of ''Methanobrevibacter'' species. == Recipe == {| class="wikitable" |'''Composition per 100 mL''' |'''Amount''' |- |''Modified Rich medium for Methanobrevibacter (MMSM)'' | |- |Mineral 1 |3.3 mL |- |Mineral 2 |3.3 mL |- |Yeast extract |0.2 g |- |Tryptone |0.2 g |- |Na Acetate·3H<sub>2</s..."</p> <p><b>New page</b></p><div><br /> == Description ==<br /> Modified ''Methanobrevibacter smithii'' and ''Methanospheara stadtmanae'' medium (MMSM) is a rich, undefined medium commonly used for the anaerobic cultivation of ''Methanobrevibacter'' species.<br /> <br /> == Recipe ==<br /> {| class="wikitable"<br /> |'''Composition per 100 mL'''<br /> |'''Amount''' <br /> |-<br /> |''Modified Rich medium for Methanobrevibacter (MMSM)''<br /> | <br /> |-<br /> |Mineral 1<br /> |3.3 mL<br /> |-<br /> |Mineral 2<br /> |3.3 mL<br /> |-<br /> |Yeast extract<br /> |0.2 g<br /> |-<br /> |Tryptone<br /> |0.2 g<br /> |-<br /> |Na Acetate·3H<sub>2</sub>O (150 g/L)<br /> |2 mL<br /> |-<br /> |Fe(NH<sub>4</sub>)<sub>2</sub>(SO<sub>4</sub>)<sub>2</sub>·6H<sub>2</sub>O (2 g/L)<br /> |1 mL<br /> |-<br /> |Rezasurin (1 g/L)<br /> |0.1 mL<br /> |-<br /> |Trace vitamins<br /> |1 mL<br /> |-<br /> |Trace minerals<br /> |1 mL<br /> |-<br /> |NaHCO<sub>3</sub><br /> |0.5 g<br /> |-<br /> |Rumen fluid<br /> |3 mL<br /> |-<br /> |NH<sub>4</sub>Cl<br /> |0.1 g<br /> |-<br /> |L-Cysteine hydrochloride·H<sub>2</sub>O<br /> |0.05 g<br /> |-<br /> |Na<sub>2</sub>S·9H<sub>2</sub>O<br /> |0.1 mL<br /> |-<br /> |DTT<br /> |0.05 g<br /> |-<br /> |ddH<sub>2</sub>O<br /> |85 mL<br /> |-<br /> |''Modification of MMSM for growth on formate''<br /> |<br /> |-<br /> |Add sodium formate<br /> |1.5 g<br /> |-<br /> |Add glycylglycine buffer, 1 M<br /> |20 mL<br /> |-<br /> |ddH<sub>2</sub>O<br /> |65 mL<br /> |}<br /> Note: some suppliers may replace up to 10 g of yeast extract with meat extract<br /> <br /> === Directions ===<br /> Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.<br /> <br /> == Sources ==<br /> <br /> * [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab)</div>

Nanashao