M2GSC Medium - Revision history

Madachi: /* Method */

2026-10-30T17:11:29Z

Method

← Older revision		Revision as of 17:11, 30 October 2026
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	== ~~Method~~ ==		== Direction ==

	* Weigh all ingredients as listed into a Pyrex conical glass flask, with the exception of cysteine.		* Weigh all ingredients as listed into a Pyrex conical glass flask, with the exception of cysteine.

Madachi: /* Method */

2026-10-29T22:09:38Z

Method

← Older revision		Revision as of 22:09, 29 October 2026
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	== Method ==		== Method ==
	~~Weigh all ingredients as listed into a Pyrex conical glass flask, with the exception of cysteine.~~

	Add mineral solutions, rumen fluid, resazurin and water.		* Weigh all ingredients as listed into a Pyrex conical glass flask, with the exception of cysteine.
			* Add mineral solutions, rumen fluid, resazurin and water.
	Microwave flask on high until rapidly boiling.		* Microwave flask on high until rapidly boiling.
			* Remove flask from microwave and bubble with a gentle stream of CO<sub>2</sub> until cool. Slowly add cysteine and then flush headspace only as bubble can change pH.
	Remove flask from microwave and bubble with a gentle stream of CO<sub>2</sub> until cool. Slowly add cysteine and then flush headspace only as bubble can change pH.		* Dispense M2GSC medium in 9.5 ml volumes into CO<sub>2</sub> flushed Hungate tubes.
			* Butyl rubber stoppers should be placed in the neck of tubes while withdrawing the CO<sub>2</sub> sparger and the tubes sealed with screw caps.
	Dispense M2GSC medium in 9.5 ml volumes into CO<sub>2</sub> flushed Hungate tubes.		* Autoclave tubes at 121ºC for 15 min. on same day as prepared.

	Butyl rubber stoppers should be placed in the neck of tubes while withdrawing the CO<sub>2</sub> sparger and the tubes sealed with screw caps.

	Autoclave tubes at 121ºC for 15 min. on same day as prepared.

	== Notes ==		== Notes ==

Madachi: /* Method */

2026-10-29T22:08:49Z

Method

← Older revision		Revision as of 22:08, 29 October 2026
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	Add mineral solutions, rumen fluid, resazurin and water.		Add mineral solutions, rumen fluid, resazurin and water.

	Microwave flask on high until rapidly boiling		Microwave flask on high until rapidly boiling.

	Remove flask from microwave and bubble with a gentle stream of CO<sub>2</sub> until cool. Slowly add cysteine and then flush headspace only as bubble can change pH.		Remove flask from microwave and bubble with a gentle stream of CO<sub>2</sub> until cool. Slowly add cysteine and then flush headspace only as bubble can change pH.

Madachi: /* Recipe */

2026-10-29T22:08:27Z

Recipe

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 == Description ==
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+M2GSC medium is based on a modified M2 medium [1] supplemented with glucose, soluble starch, cellobiose, and rumen fluid in an anaerobic broth.
 == Recipe ==
-add here
+'''M2GSC medium composition'''
-== Direction ==
-add here
+'''Mineral solution 1'''
 == Notes ==
-add here
+Note: for rumen fluid
 == Sources ==

Madachi at 21:44, 29 October 2026

2026-10-29T21:44:44Z

← Older revision		Revision as of 21:44, 29 October 2026
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	== Description ==		== Description ==
			add here

	== Recipe ==		== Recipe ==
			add here

	==~~= Directions =~~==		== Direction ==
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	== Notes ==		== Notes ==
			add here

	== Sources ==		== Sources ==
	~~[1] Caldwell DR, Bryant MP. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol 14:794–801.~~

Madachi: Created page with " == Description == == Recipe == === Directions === == Notes == == Sources == [1] Caldwell DR, Bryant MP. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol 14:794–801."

2026-10-29T21:43:44Z

Created page with " == Description == == Recipe == === Directions === == Notes == == Sources == [1] Caldwell DR, Bryant MP. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol 14:794–801."

New page

== Description ==

== Recipe ==

=== Directions ===

== Notes ==

== Sources ==
[1] Caldwell DR, Bryant MP. 1966. Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl Microbiol 14:794–801.