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Lachnospiraceae bacterium ND2006 - Revision history
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Billkelleydonvis: Introduction and external links
2026-10-30T03:22:11Z
<p>Introduction and external links</p>
<p><b>New page</b></p><div><br />
== Introduction ==<br />
'''''Lachnospiraceae'' bacterium ND2006'''<br />
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''Lachnospiraceae'' bacterium ND2006 (DSM 121559) was isolated from the plant-adherent fraction of bovine rumen contents in NZ [1, cow D]. ND2006 is Gram negative, non-motile and can ferment a wide range of carbohydrates. Growth on glucose produced lactate with lower amounts of acetate, butyrate, formate and hydrogen. In GTDB ND2006 has been assigned to an uncharacterized genus (NK4A144) within the family ''Lachnospiraceae''.<br />
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The ND2006 genome encodes a CRISPR-Cas12a system (LbCas12a/LbCpf1) [2, 3] that has been widely used for gene editing.<br />
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The draft genome for ND2006 (3,945,387 bp) was generated through the Hungate1000 project with JGI [4].<br />
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NCBI: ''Lachnospiraceae'' bacterium.<br />
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GTDB: NK4A144 sp900114405.<br />
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Risk group: 1.<br />
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== Global Methane Hub Metadata ==<br />
{| class="wikitable"<br />
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|Origin:<br />
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|Sequenced by:<br />
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|Assembled by:<br />
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|Methylome called by:<br />
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|Methyltransferases cloned by:<br />
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|MIC and Culturing Conditions by:<br />
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|Transformation by:<br />
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== Genome ==<br />
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== DefenseFinder Results ==<br />
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== Methylome ==<br />
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== Methylating E. coli Strains ==<br />
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== Culturing Conditions ==<br />
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== Antibiotic MIC Tests ==<br />
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== Protocols ==<br />
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== Transformation Results ==<br />
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== External Links ==<br />
[1] Noel S. 2013. Cultivation and community composition analysis of plant-adherent rumen bacteria. PhD dissertation. Massey University, Palmerston North, New Zealand. <nowiki>https://mro.massey.ac.nz/server/api/core/bitstreams/bcfbe4cb-19a9-40c0-b5e8-61f9ddb90ce0/content</nowiki>.<br />
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[2] Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. 2016. Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Nat Biotechnol 34: 869-74. doi: 10.1038/nbt.3620.<br />
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[3] <nowiki>https://undark.org/2025/03/05/digital-genetic-data-ownership/</nowiki><br />
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[4] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.</div>
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