Butyrivibrio hungatei MB2003 - Revision history

Yuqili at 17:13, 30 October 2026

2026-10-30T17:13:38Z

← Older revision		Revision as of 17:13, 30 October 2026
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	== Methylating ''E. coli'' ==		== Methylating ''E. coli'' ==
			add here

	== Culturing Conditions ==		== Culturing Conditions ==

Yuqili at 17:13, 30 October 2026

2026-10-30T17:13:10Z

← Older revision		Revision as of 17:13, 30 October 2026
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	\|97.76%		\|97.76%
	\|}		\|}

			== Methylating ''E. coli'' ==

	== Culturing Conditions ==		== Culturing Conditions ==

Yuqili at 17:08, 30 October 2026

2026-10-30T17:08:37Z

← Older revision		Revision as of 17:08, 30 October 2026
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	== Culturing Conditions ==		== Culturing Conditions ==
	''Butyrivibrio hungatei'' MB2003 grows well in [[M2GSC Medium\|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in [[M2GSC]] medium. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.		''Butyrivibrio hungatei'' MB2003 grows well in [[M2GSC Medium\|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in [[M2GSC Medium\|M2GSC]] medium. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==

Yuqili at 17:07, 30 October 2026

2026-10-30T17:07:57Z

← Older revision		Revision as of 17:07, 30 October 2026
Line 57:		Line 57:

	== Culturing Conditions ==		== Culturing Conditions ==
	''Butyrivibrio hungatei'' MB2003 grows well in M2GSC medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in M2GSC medium. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.		''Butyrivibrio hungatei'' MB2003 grows well in [[M2GSC Medium\|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cells can be grown overnight in [[M2GSC]] medium. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==

Yuqili at 23:21, 28 October 2026

2026-10-28T23:21:36Z

← Older revision		Revision as of 23:21, 28 October 2026
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			[[File:MB2003SEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Butyrivibrio hungatei'' MB2003. Source: Yuqi Li]]

	== Introduction ==		== Introduction ==

Yuqili: /* Antibiotic MIC Tests */

2026-10-28T23:17:34Z

Antibiotic MIC Tests

← Older revision		Revision as of 23:17, 28 October 2026
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	\|-		\|-
	\|Ampicillin		\|Ampicillin
	\|0.52		\|0.91
	\|-		\|-
	\|Kanamycin		\|Kanamycin
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	\|-		\|-
	\|Chloramphenicol		\|Chloramphenicol
	\|~~Resistant (>200 μg/mL)~~		\|0.59
	\|-		\|-
	\|Tetracycline		\|Tetracycline
	\|~~0.20~~		\|Resistant (>200 μg/mL)
	\|-		\|-
	\|Gentamicin		\|Gentamicin
	\|~~Resistant (>200 μg/mL)~~		\|2.08
	\|-		\|-
	\|Streptomycin		\|Streptomycin
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	\|-		\|-
	\|Erythromycin		\|Erythromycin
	\|4.3		\|4.18
	\|}		\|}

Yuqili at 23:13, 28 October 2026

2026-10-28T23:13:22Z

@@ Line 53: / Line 53: @@
 |97.76%
 |}
 == External Links ==

Yuqili at 22:59, 28 October 2026

2026-10-28T22:59:49Z

← Older revision		Revision as of 22:59, 28 October 2026
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	Risk group: 1		Risk group: 1

			== Global Methane Hub Metadata ==
			{\| class="wikitable"
			\|Origin:
			\|New Zealand, Kelly Lab
			\|-
			\|Sequenced by:
			\|Yuqi Li
			\|-
			\|Assembled by:
			\|William G. Alexander
			\|-
			\|Methylome called by:
			\|William G. Alexander
			\|-
			\|Methyltransferases cloned by:
			\|Roy Rodriguez Carrero
			\|-
			\|MIC and Culturing Conditions by:
			\|Yuqi Li
			\|}

			== Genome ==
			[[File:MB2003.png\|none\|thumb\|Circos plot of ''B. hungatei'' MB2003. Source: Yuqi Li]]

	== Methylome ==		== Methylome ==

Billkelleydonvis: Introduction and external links

2026-10-20T22:12:26Z

Introduction and external links

@@ Line 1: / Line 1: @@
 == Methylome ==
 {| class="wikitable"
@@ Line 13: / Line 29: @@
 |97.76%
 |}

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |C(6mA)CNNNNNNTGTT/ AAC(6mA)NNNNNNGTG |94.54%/94.83% |- |ATTA(6mA)T |97.76% |}"

2026-09-30T14:49:07Z

Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |C(6mA)CNNNNNNTGTT/ AAC(6mA)NNNNNNGTG |94.54%/94.83% |- |ATTA(6mA)T |97.76% |}"

New page

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|C(6mA)CNNNNNNTGTT/

AAC(6mA)NNNNNNGTG
|94.54%/94.83%
|-
|ATTA(6mA)T
|97.76%
|}