Acetitomaculum ruminis 139BT - Revision history

Yuqili at 18:26, 30 October 2026

2026-10-30T18:26:33Z

← Older revision		Revision as of 18:26, 30 October 2026
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	== Introduction ==		== Introduction ==
	'''''Acetitomaculum ruminis'' ~~139Bᵀ~~''' (DSM 5522, ATCC 43876) is a strictly anaerobic, Gram-positive, rod-shaped bacterium belonging to the family Lachnospiraceae. It was originally isolated from the rumen of cattle and first described by Greening and Leedle in 1989 [1]. The strain 139B<sup>T</sup> is designated as the type strain of ''A. ruminis'' [1, 2].		'''''Acetitomaculum ruminis'' 139B<sup>T</sup>''' (DSM 5522, ATCC 43876) is a strictly anaerobic, Gram-positive, rod-shaped bacterium belonging to the family Lachnospiraceae. It was originally isolated from the rumen of cattle and first described by Greening and Leedle in 1989 [1]. The strain 139B<sup>T</sup> is designated as the type strain of ''A. ruminis'' [1, 2].

	''A. ruminis'' is an acetogenic bacterium capable of utilizing hydrogen (H<sub>2</sub>) and carbon dioxide (CO<sub>2</sub>) to produce acetate through the Wood–Ljungdahl pathway [1, 3]. It can also utilize organic substrates for growth. As a hydrogen-consuming acetogen, ''A. ruminis'' may compete with methanogenic archaea for hydrogen in the rumen. However, methanogenesis is generally considered the dominant hydrogen disposal pathway under typical rumen conditions [3, 4].		''A. ruminis'' is an acetogenic bacterium capable of utilizing hydrogen (H<sub>2</sub>) and carbon dioxide (CO<sub>2</sub>) to produce acetate through the Wood–Ljungdahl pathway [1, 3]. It can also utilize organic substrates for growth. As a hydrogen-consuming acetogen, ''A. ruminis'' may compete with methanogenic archaea for hydrogen in the rumen. However, methanogenesis is generally considered the dominant hydrogen disposal pathway under typical rumen conditions [3, 4].
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	== Methylating E. coli Strains ==		== Methylating ''E. coli'' Strains ==

	== Culturing Conditions ==		== Culturing Conditions ==
			''Acetitomaculum ruminis'' 139B<sup>T</sup> can be cultivated in [[M2GSC Medium\|M2GSC]] medium at 37 °C under strictly anaerobic conditions. Cultures are maintained in an anaerobic chamber containing 95% CO<sub>2</sub> and 5% H<sub>2</sub>.

	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==
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	== External Links ==		== External Links ==
			[1] Greening RC, Leedle JAZ. 1989. Enrichment and isolation of ''Acetitomaculum ruminis'', gen. nov., sp. nov.: acetogenic bacteria from the bovine rumen. Archives of Microbiology 151: 399–406. doi: 10.1007/BF00416597.

			[2] DSMZ – German Collection of Microorganisms and Cell Cultures. ''Acetitomaculum ruminis'' DSM 5522. <nowiki>https://www.dsmz.de/collection/catalogue/details/culture/DSM-5522</nowiki>.

			[3] Ragsdale SW, Pierce E. 2008. Acetogenesis and the Wood–Ljungdahl pathway of CO<sub>2</sub> fixation. Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics 1784: 1873–1898. doi: 10.1016/j.bbapap.2008.08.012.

			[4] Ungerfeld EM. 2020. Metabolic hydrogen flows in rumen fermentation: principles and possibilities of interventions. Frontiers in Microbiology 11: 589. doi: 10.3389/fmicb.2020.00589.

			[5] Seshadri R, Leahy SC, Attwood GT, et al. 2018. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnology 36: 359–367. doi: 10.1038/nbt.4110.

Yuqili: /* Introduction */

2026-10-30T18:19:26Z

Introduction

← Older revision		Revision as of 18:19, 30 October 2026
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	== Introduction ==		== Introduction ==
			'''''Acetitomaculum ruminis'' 139Bᵀ''' (DSM 5522, ATCC 43876) is a strictly anaerobic, Gram-positive, rod-shaped bacterium belonging to the family Lachnospiraceae. It was originally isolated from the rumen of cattle and first described by Greening and Leedle in 1989 [1]. The strain 139B<sup>T</sup> is designated as the type strain of ''A. ruminis'' [1, 2].

			''A. ruminis'' is an acetogenic bacterium capable of utilizing hydrogen (H<sub>2</sub>) and carbon dioxide (CO<sub>2</sub>) to produce acetate through the Wood–Ljungdahl pathway [1, 3]. It can also utilize organic substrates for growth. As a hydrogen-consuming acetogen, ''A. ruminis'' may compete with methanogenic archaea for hydrogen in the rumen. However, methanogenesis is generally considered the dominant hydrogen disposal pathway under typical rumen conditions [3, 4].

			The genome sequence of ''A. ruminis'' 139B<sup>T</sup> is available through the Hungate1000 project, which provides genomic resources for understanding the metabolic capabilities and ecological roles of rumen microorganisms [5]. Its acetogenic metabolism makes this bacterium particularly interesting for studying rumen hydrogen utilization and potential alternative pathways to methane formation.

			LPSN: ''Acetitomaculum ruminis'' (Greening and Leedle, 1989).

			NCBI: ''Acetitomaculum ruminis''.

			GTDB: ''Acetitomaculum ruminis''.

			Type strain: 139B<sup>T</sup> (= DSM 5522 = ATCC 43876).

	== Global Methane Hub Metadata ==		== Global Methane Hub Metadata ==

Yuqili: /* Antibiotic MIC Tests */

2026-10-28T23:54:06Z

Antibiotic MIC Tests

← Older revision		Revision as of 23:54, 28 October 2026
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	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==
	[[File:139BMIC.png~~\|none~~\|thumb\|MIC tests of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]		[[File:139BMIC.png\|thumb\|MIC tests of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li\|left]]
	{\| class="wikitable"		{\| class="wikitable"
	!Antibiotic		!Antibiotic
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	\|-		\|-
	\|Ampicillin		\|Ampicillin
	\|~~0.52~~		\|Resistant (>200 μg/mL)
	\|-		\|-
	\|Kanamycin		\|Kanamycin
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	\|-		\|-
	\|Chloramphenicol		\|Chloramphenicol
	\|~~Resistant (>200 μg/mL)~~		\|1.03
	\|-		\|-
	\|Tetracycline		\|Tetracycline
	\|0.20		\|0.19
	\|-		\|-
	\|Gentamicin		\|Gentamicin
	\|~~Resistant (>200 μg/mL)~~		\|4.75
	\|-		\|-
	\|Streptomycin		\|Streptomycin
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	\|-		\|-
	\|Erythromycin		\|Erythromycin
	\|4.3		\|2.67
	\|}		\|}

Yuqili: /* Antibiotic MIC Tests */

2026-10-28T23:52:51Z

Antibiotic MIC Tests

← Older revision		Revision as of 23:52, 28 October 2026
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	== Antibiotic MIC Tests ==		== Antibiotic MIC Tests ==
			[[File:139BMIC.png\|none\|thumb\|MIC tests of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]
			{\| class="wikitable"
			!Antibiotic
			!Concentration ('''μ'''g/mL)
			\|-
			\|Ampicillin
			\|0.52
			\|-
			\|Kanamycin
			\|Resistant (>200 μg/mL)
			\|-
			\|Chloramphenicol
			\|Resistant (>200 μg/mL)
			\|-
			\|Tetracycline
			\|0.20
			\|-
			\|Gentamicin
			\|Resistant (>200 μg/mL)
			\|-
			\|Streptomycin
			\|Resistant (>200 μg/mL)
			\|-
			\|Erythromycin
			\|4.3
			\|}

	== Protocols ==		== Protocols ==

Yuqili: /* ☂Introduction */

2026-10-28T23:50:51Z

☂Introduction

← Older revision		Revision as of 23:50, 28 October 2026
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	[[File:139BSEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]		[[File:139BSEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]

	== ~~☂Introduction~~ ==		== Introduction ==

	== Global Methane Hub Metadata ==		== Global Methane Hub Metadata ==

Yuqili at 23:50, 28 October 2026

2026-10-28T23:50:29Z

← Older revision		Revision as of 23:50, 28 October 2026
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	== ~~Introduction~~ ==		[[File:139BSEM.jpg\|thumb\|Scanning electron microscopy (SEM) image of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]

			== ☂Introduction ==

	== Global Methane Hub Metadata ==		== Global Methane Hub Metadata ==

Yuqili: /* Methylome */

2026-10-28T23:48:24Z

Methylome

← Older revision		Revision as of 23:48, 28 October 2026
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	== Methylome ==		== Methylome ==
			{\| class="wikitable"
			!Motif
			!Percent methylated
			\|-
			\|CTGC(6mA)G
			\| align="right" \|95.68%
			\|-
			\|TA(6mA)GNNNNNNTCC
			\| align="right" \|95.61%
			\|-
			\|GG(6mA)NNNNNNCTTA
			\| align="right" \|96.1%
			\|-
			\|G(6mA)TGNNNNNNTCC
			\| align="right" \|84.95%
			\|-
			\|GG(6mA)NNNNNNCATT
			\| align="right" \|92.38%
			\|-
			\|G(6mA)TCG
			\| align="right" \|89.82%
			\|-
			\|A(6mA)TGNNNNNNTCC
			\| align="right" \|96.19%
			\|-
			\|GG(6mA)NNNNNNCATT
			\| align="right" \|90.36%
			\|-
			\|GAT(5mC)
			\| align="right" \|32.66%
			\|}

	== Methylating E. coli Strains ==		== Methylating E. coli Strains ==

Yuqili: /* Global Methane Hub Metadata */

2026-10-28T23:45:05Z

Global Methane Hub Metadata

← Older revision		Revision as of 23:45, 28 October 2026
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	\|+		\|+
	\|Origin:		\|Origin:
	\|		\|Illinois, Mackie Lab
	\|-		\|-
	\|Sequenced by:		\|Sequenced by:
	\|		\|Yuqi Li
	\|-		\|-
	\|Assembled by:		\|Assembled by:
	\|		\|William G. Alexander
	\|-		\|-
	\|Methylome called by:		\|Methylome called by:
	\|		\|William G. Alexander
	\|-
	~~\|Methyltransferases cloned by:~~
	\|
	\|-		\|-
	\|MIC and Culturing Conditions by:		\|MIC and Culturing Conditions by:
	\|		\|Yuqi Li
	\|-
	~~\|Transformation by:~~
	\|
	\|}		\|}

	== Genome ==		== Genome ==
			[[File:139BGenome.png\|none\|thumb\|Circos plot of ''Acetitomaculum ruminis'' 139B. Source: Yuqi Li]]

	== DefenseFinder Results ==		== DefenseFinder Results ==

Yuqili: Created page with " == Introduction == == Global Methane Hub Metadata == {| class="wikitable" |+ |Origin: | |- |Sequenced by: | |- |Assembled by: | |- |Methylome called by: | |- |Methyltransferases cloned by: | |- |MIC and Culturing Conditions by: | |- |Transformation by: | |} == Genome == == DefenseFinder Results == == Methylome == == Methylating E. coli Strains == == Culturing Conditions == == Antibiotic MIC Tests == == Protocols == == Transformation Results == == External Link..."

2026-10-28T23:40:03Z

Created page with " == Introduction == == Global Methane Hub Metadata == {| class="wikitable" |+ |Origin: | |- |Sequenced by: | |- |Assembled by: | |- |Methylome called by: | |- |Methyltransferases cloned by: | |- |MIC and Culturing Conditions by: | |- |Transformation by: | |} == Genome == == DefenseFinder Results == == Methylome == == Methylating E. coli Strains == == Culturing Conditions == == Antibiotic MIC Tests == == Protocols == == Transformation Results == == External Link..."

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== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
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|Origin:
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|Sequenced by:
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|Assembled by:
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|Methylome called by:
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|Methyltransferases cloned by:
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|MIC and Culturing Conditions by:
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|Transformation by:
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== Genome ==

== DefenseFinder Results ==

== Methylome ==

== Methylating E. coli Strains ==

== Culturing Conditions ==

== Antibiotic MIC Tests ==

== Protocols ==

== Transformation Results ==

== External Links ==