View source for Olsenella sp. KH2P3


== Introduction ==
'''''Tractidigestivibacter'' sp. KH2P3'''

''Tractidigestivibacter'' sp. KH2P3 was isolated from bovine rumen contents in NZ using KH medium which was supplemented with haemin and vitamin K [1]. KH2P3 is a non-motile, non-spore forming Gram positive, anaerobe which produces D-lactate as the main product from glucose fermentation. KH2P3 is a high G+C lactic acid bacterium and while originally identified as ''Olsenella'' it was subsequently assigned to the genus ''Tractidigestivibacter'' within the family ''Atopobiaceae'' [2].

''Tractidigestivibacter'' species encode glycyl radical enzyme (GRE) superfamily enzymes that catalyse reactions rarely found in other bacteria, such as aromatic amino acid decarboxylases that enable ''T. scatoligenes'' to produce skatole and p-cresol from tryptophan and tyrosine [3]. KH2P3 encodes two arylacetate decarboxylase gene clusters.

The draft genome for KH2P3 (2,421,020 bp) was generated through the Hungate1000 project with JGI [1].

NCBI: ''Olsenella'' sp.

GTDB: ''Tractidigestivibacter'' sp900119625

Risk group: 1.

== Global Methane Hub Metadata ==
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== Genome ==

== DefenseFinder Results ==

== Methylome ==

== Methylating E. coli Strains ==

== Culturing Conditions ==

== Antibiotic MIC Tests ==

== Protocols ==

== Transformation Results ==

== External Links ==
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

[2] Zgheib R, Anani H, Meng MM, Mailhe M, Ricaboni D, Morand A, Caputo A, Traore SI, Fontanini A, Armstrong N, Raoult D, Fournier PE, 2021. New human-associated species of the family ''Atopobiaceae'' and proposal to reclassify members of the genus ''Olsenella''. Int J Syst Evol Microbiol 71. doi: 10.1099/ijsem.0.004819.

[3] Li X, Jensen RL, Højberg O, Canibe N, Jensen BB, 2015. ''Olsenella scatoligenes'' sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces. Int J Syst Evol Microbiol 65, 1227-1233. doi: 10.1099/ijs.0.000083.