View source for Anaerovibrio lipolyticus 5ST


== Introduction ==
'''Anaerovibrio lipolyticus 5S<sup>T</sup>'''

Anaerovibrio lipolyticus 5S<sup>T</sup> (DSM 3074) is an anaerobic Gram-negative bacterium isolated from sheep rumen contents in Scotland (UK) [1] and originally named Anaerovibrio lipolytica [3]. It is the type species of the genus Anaerovibrio within the family Selenomonadaceae and the type stain of the A. lipolyticus species [2]. Ribose, fructose and lactate were used as growth substrates and glycerol was fermented to propionate, and succinate [4]. It is regarded as one of the main lipolytic species in the rumen and lipases have been characterized [5].

A draft genome for A. lipolyticus 5S (2,781,134 bp) was generated by the JGI GEBA project.

LPSN: Anaerovibrio lipolyticus corrig. Hungate 1966

NCBI: Anaerovibrio lipolyticus

GTDB: Anaerovibrio lipolyticus

Risk group: 1

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|DSMZ
|-
|Sequenced by:
|Makoto Adachi
|-
|Assembled by:
|William G. Alexander
|-
|Methylome called by:
|William G. Alexander
|-
|Methyltransferases cloned by:
|
|-
|MIC and Culturing Conditions by:
|
|-
|Transformation by:
|
|}


== Genome ==
[[File:NVJKVF 1 1 1 circos.png|center|thumb|301x301px|Circos plot of ''A. lipolyticus'' 5S<sup>T</sup>. Source: Makoto Adachi]]

== DefenseFinder Results ==
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== Methylome ==
{| class="wikitable"
|'''Motif'''
|'''Percent methylated'''
|-
|G(6mA)TC
| align="right" |99.94
|-
|GATGA(6mA)
| align="right" |100
|-
|GAA(6mA)CC
| align="right" |98.2
|-
|GCCC(6mA)G
| align="right" |98.78
|-
|G(6mA)CNNNNNTAAC
| align="right" |100
|-
|GTT(6mA)NNNNNGTC
| align="right" |93.96
|-
|G(6mA)GNNNNCTAA
| align="right" |98.89
|-
|TT(6mA)GNNNNCTC
| align="right" |85.56
|-
|CAG(4mC)TG
| align="right" |96.22
|}

== Methylating ''E. coli'' ==
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== Culturing Conditions ==
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== Antimicrobial MIC Tests ==
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== Protocols ==
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== Transformation Results ==
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== External Links ==
[1] Hobson PN, Mann SO. 1961. The isolation of glycerol fermenting and lipolytic bacteria from the rumen of the sheep. J Gen Microbiol 25: 227–240. doi: 10.1099/00221287-25-2-227.

[2] <nowiki>https://bacdive.dsmz.de/strain/17117</nowiki>.

[3] Hungate RE. 1966. The rumen and its microbes. Academic Press, New York, USA.

[4] Prins RA, Lankhorst A, van der Meer P, Van Nevel CJ. 1975. Some characteristics of Anaerovibrio lipolytica a rumen lipolytic organism. Antonie Van Leeuwenhoek 41: 1-11. doi: 10.1007/BF02565031.

[5] Privé F, Kaderbhai NN, Girdwood S, Worgan HJ, Pinloche E, Scollan ND, Huws SA, Newbold CJ.2013. Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5S<sup>T</sup>. PLoS One 8 : e69076. doi: 10.1371/journal.pone.0069076.