View source for Methanobrevibacter olleyae YLM1


== Introduction ==
''Methanobrevibacter olleyae'' YLM1 was isolated from the rumen of a lamb<ref>Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: [https://www.sciencedirect.com/science/article/pii/S1075996404000496?via%3Dihub 10.1016/j.anaerobe.2004.05.003]. </ref>. Although initially identified as ''Methanobrevibacter'' sp.  or ''Methanobrevibacter smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the ''Methanocatella (Methanobrevibacter) millerae'' type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species<ref>Kelly WJ, Li D, Lambie SC, Cox F, Attwood GT, Altermann E, Leahy SC. Draft Genome Sequence of the Rumen Methanogen ''Methanobrevibacter olleyae'' YLM1. Genome Announc. 2016 Apr 7;4(2):e00232-16. doi: [https://journals.asm.org/doi/10.1128/genomea.00232-16 10.1128/genomeA.00232-16].</ref>.  Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub.

== Global Methane Hub Metadata ==
{| class="wikitable"
|'''Origin:'''
|Lucidome Bio, NZ
|-
|'''Sequenced by:'''
|Plasmidsaurus, ONT + Illumina
|-
|'''Assembled by:'''
|Nana Shao
|-
|'''Methylome called by:'''
|William G. Alexander
|-
|'''Methyltransferases cloned by:'''
|Roy Rodriguez Carrero
|-
|'''MIC and Culturing conditions by:'''
|Nana Shao
|-
|'''Transformation by:'''
|
|}

== Genome ==
add here

== DefenseFinder Results ==
add here

== Methylome ==
add here

== Methylating ''E. coli'' ==
add here

== Culturing Conditions ==
''M. millerae'' SM9 is cultivated in [[Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM)|Modified ''Methanobrevibacter smithii'' and ''Methanosphaera stadtmanae'' medium (MMSM)]] at 37°C. To ensure good gas transfer to the media, tubes are incubated on their sides (or at a shallow angle so that the media does not contact the stoppers) with gentle shaking at 50 rpm and 207 kPa of H<sub>2</sub>/CO<sub>2</sub> (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same pressure of H<sub>2</sub>/CO<sub>2</sub> as an energy substrate for 3-7 days.

== Antimicrobial MIC Tests ==
<gallery>
File:Puromycin_MIC_determination_in_M._millerae_SM9._Source-_Dr._Nana_Shao..jpg|Puromycin MIC determination in ''M. millerae'' SM9. Source: Dr. Nana Shao
</gallery>
{| class="wikitable"
|+
! rowspan="2" |Antibiotics
! colspan="2" |Concentration
|-
|'''Liquid'''
|'''Solid'''
|-
|Puromycin
|
|
|-
|nourseothricin
|
|μM
|}

== Protocols ==
add here

== Transformation Results ==
add here

== External Links ==
<references />