View source for Selenomonas ruminantium HD4


== Introduction ==
'''''Pseudoselenomonas ruminantium'' HD<sub>4</sub>'''

''Pseudoselenomonas ruminantium'' HD<sub>4</sub> was isolated from bovine rumen contents in the USA [1] and characterised along with other ''Selenomonas'' strains [2]. HD<sub>4</sub> is an anaerobic bacterium that ferments a broad range of carbohydrates and produces primarily propionate, and acetate. Lactate is fermented [2] and HD<sub>4</sub> can reduce nitrate, sulfate and fumarate.

The draft genome for HD<sub>4</sub> (3,116,786 bp) was generated through the Hungate1000 project with JGI [3]. A 4.8 kb plasmid has been reported from HD<sub>4</sub> [4] and a 4,899 bp plasmid was predicted from the draft genome sequence. Resequencing gave a genome of 3,180,636 bp consisting of a 2,987,729 bp chromosome and a 192,907 bp plasmid.

NCBI: ''Pseudoselenomonas ruminantium''.

GTDB: ''Selenomonas_A ruminantium_D''. HD<sub>4</sub> belongs in a different species group than the type strain of ''P. ruminantium'' (GA192, DSM 2150).

Risk group: 1.

== Global Methane Hub Metadata ==
{| class="wikitable"
|Origin:
|United States of America, Macike lab
|-
|Sequenced by:
|Who did that...?
|-
|Assembled by:
|William G. Alexander
|-
|Methylome called by:
|William G. Alexander
|-
|Methyltransferases cloned by:
|Jill Walton
|-
|MIC and Culturing Conditions by:
|Makoto Adachi
|-
|Transformation by:
|
|}

== Genome ==
add here

== DefenseFinder Results ==
add here

== Methylome ==
add here

== Methylating ''E. coli'' ==
add here

== Culturing Conditions ==
add here

== Antimicrobial MIC Tests ==
add here

== Protocols ==
add here

== Transformation Results ==
add here

== External Links ==
[1] Huhtanen C N, Gall LS. 1953. Rumen organisms I. Curved rods and a related rod type. J Bacteriol 65, 548-553. doi: 10.1128/jb.65.5.548-553.1953.

[2] Bryant MP. 1956. The characteristics of strains of ''Selenomonas'' isolated from bovine rumen contents. J Bacteriol 72: 162-7. doi: 10.1128/jb.72.2.162-167.1956.

[2] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

[4] Martin SA, Dean RG. 1989. Characterization of a plasmid from the ruminal bacterium ''Selenomonas ruminantium''. Appl Environ Microbiol 55: 3035–3038. doi: 10.1128/aem.55.12.3035-3038.1989.