View source for Selenomonas ruminantium GA192

Bacillota (firmicutes)- Negativicutes - Selenomonadales - Selenomonadaceae

== Introduction ==
'''''Pseudoselenomonas ruminantium'' GA192<sup>T</sup>'''

''Pseudoselenomonas ruminantium'' GA192<sup>T</sup> (DSM 2150) formerly ''Selenomonas ruminantium'' was isolated from bovine rumen contents from an animal fed fresh alfalfa in the USA [1]. GA192 is the type species of the genus ''Pseudoselenomonas'' within the family ''Selenomonadaceae'' and the type stain of the ''P. ruminantium'' species [2, 3]. GA192 is an anaerobic bacterium that ferments a broad range of carbohydrates and produces primarily propionate, lactate, and CO₂ [1, 2]. Lactate is not fermented [1], sulfate and fumarate but not nitrate are reduced.

The draft genome for GA192 (3,387,070 bp) was generated through the Hungate1000 project with JGI [4]. A20,067 bp plasmid has been predicted from the genome sequence.

LPSN: ''Pseudoselenomonas ruminantium'' (Certes 1889) Heng and Kittelmann 2026

NCBI: ''Pseudoselenomonas ruminantium''.

GTDB: ''Selenomonas_A ruminantium''.

Risk group: 1.

== Genome ==

== Methylome ==

{| class="wikitable"
|+ Methylome Motifs
|-
! motif !! # site methylated !! total sites !! % methylated
|-
| G(6mA)TC || Example || Example || Example
|-
| G(6mA)CNNNNNCTC/<br />G(6mA)GNNNNNGTC || Example || Example || Example
|-
| GA(6mA)YNNNNNAATC/<br />G(6mA)TTNNNNRTTC || Example || Example || Example
|-
| CC(6mA)GNNNNNTCG/<br />CG(6mA)NNNNNCTGG || Example || Example || Example
|-
| GA(6mA)GNNNNNCTC/<br />G(6mA)GNNNNNCTTC || Example || Example || Example
|-
| GA(6mA)CNNNNNACT/<br />(6mA)GTNNNNNGTTC || Example || Example || Example
|}

== ''E.coli'' methylation strain == 
Completed, AG22058

== Antibiotic MIC tests ==
==== MIC test strip result ====
{| class="wikitable"
|+ MIC test strip result
|-
! Antibiotic !! MIC
|-
| Ampicillin || 0.016ug/ml
|-
| Tetracycline || 0.094ug/ml
|-
| Trimethoprim || 8 ug/ml
|-
| Streptomycin || 2 ug/ml
|-
| Spectinomycin || 16ug/ml
|-
| Kanamycin || 3 ug/ml
|-
| Chloramphenicol || 0.5 ug/ml
|-
| Gentamicin || 2 ug/ml
|-
| Erythromycin || 0.2ug/ml
|}

== Defensefinder prediction ==
{| class="wikitable"
|+ Defense Systems
|-
! Type / Subtype !! Mechanism !! Note
|-
| PD-Lambda-1 (PD-Lambda-1) || || 
|-
| PrrC (PrrC) || || 
|-
| RM (RM_Type_IV) || || 
|-
| Abi2 (Abi2) || || 
|-
| VP1839 (VP1839) || || 
|-
| Wadjet (Wadjet_I) || || Lacks JetD (endonuclease)
|-
| Eleos (Eleos) || || 
|-
| Cas (CAS_Class1-Subtype-I-F) || || 
|-
| SspBCDE (SspBCDE) || || Lacks SspA & SspE (endonuclease)
|-
| Cas (CAS_Class1-Subtype-III-D) || || 
|-
| AbiD (AbiD) || || 
|-
| Mokosh (Mokosh_Type_I_B) || || 
|-
| VP1851 (VP1851) || || 
|-
| Cas (CAS_Cluster) || || 
|}

== External Links ==
[1] Bryant MP. 1956. The characteristics of strains of ''Selenomonas'' isolated from bovine rumen contents. J Bacteriol 72: 162-7. doi: 10.1128/jb.72.2.162-167.1956.

[2] Heng YC, Kittelmann S. 2026. Taxonomic reclassification and expansion of the family ''Selenomonadaceae''. Int J Syst Evol Microbiol 76: 7178. doi: 10.1099/ijsem.0.007178.

[3] <nowiki>https://bacdive.dsmz.de/strain/17156</nowiki>.

[4] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.e