File:Sulfur source of M. millerae SM9.jpg
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The growth of strain SM9 exhibited no growth preference for cysteine over Na2S. Cultures supplemented with either autoclaved or filter sterilized cysteine, or with 0.4 mM autoclaved Na2S, all exhibited robust growth to high OD600 of ~1.2, whereas cultures lacking cysteine or Na2S showed markedly reduced growth. Good growth observed with filter-sterilized cysteine suggests that cells take up cysteine as a sulfur source. The sulfur source in MMSM without cysteine and sulfide has not been identified.
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