File:Antimicrobial MIC Determination in M. olleyae YLM1 in Liquid Medium.jpg

Puromycin and nourseothricin susceptibility of M. olleyae YLM1 was assessed in liquid MMSM at an initial inoculum of 2 × 10⁷ cells/mL, using the absence of a detectable increase in OD₆₀₀ throughout the observation period as the endpoint. The apparent MIC of puromycin was 30 µg/mL over approximately 650 h, with delayed growth observed at concentrations up to 20 µg/mL. The apparent MIC of nourseothricin was >80 µg/mL over approximately 550 h, as delayed growth occurred at all tested concentrations (30–80 µg/mL).