Global Methane Hub Wikipedia - User contributions [en]

Methanobrevibacter olleyae 1H5-1PT

2026-10-28T18:46:49Z

Sharik:

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexande, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|N/A
|-
|MIC and Culturing Conditions by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|Sharik Khan, University of Illinois at Urbana-Champaign
Komal Agrawal, University of Illinois at Urbana-Champaign
|}

== Genome ==
Single contig of 2,173,952 bp.

== DefenseFinder Results ==

== Methylome ==
No methylation motifs were detected in ''Methanobrevibacter olleyae'' 1H5-1PT.

== Methylating E. coli Strains ==
No ''E. coli'' methylation strain has been constructed for ''Methanobrevibacter olleyae'' 1H5-1PT.

== Culturing Conditions ==
''M. olleyae'' 1H5-1PT is a strict anaerobe and grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H2/CO2 (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .

== MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Puromycin
|
|-
|Nourseothricin
|
|-
|Neomycin
|
|-
|8-ADP
|
|-
|5-Fluorouracil
|
|-
|2-BES
|
|-
|5-FOA
|
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter boviskoreani JH1T

2026-10-28T18:46:41Z

Sharik: /* Antibiotic MIC Tests */

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexande, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|Adam Guss, Oak Ridge National Laboratory
|-
|MIC and Culturing Conditions by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|
|}

== Genome ==
Single contig of 2,084,047 bp.

== DefenseFinder Results ==

== Methylome ==
{| class="wikitable"
| valign="top" |'''#6mA modified motifs'''
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#motif'''
| valign="top" |methylCounts
| valign="top" |totalCounts
| valign="top" |%motifsMethyl
|-
| valign="top" |'''C(6mA)TCC'''
| valign="top" |2452
| valign="top" |2922
| valign="top" |83.92
|-
| valign="top" |'''GATG(6mA)T'''
| valign="top" |2170
| valign="top" |2556
| valign="top" |84.90
|-
| valign="top" |'''GG(6mA)TG'''
| valign="top" |2471
| valign="top" |2922
| valign="top" |84.57
|-
| valign="top" |'''(6mA)GGNNNNNNRTC'''
| valign="top" |1037
| valign="top" |1342
| valign="top" |77.27
|-
| valign="top" |'''G(6mA)YNNNNNNCCT'''
| valign="top" |984
| valign="top" |1342
| valign="top" |73.32
|}

== Methylating E. coli Strains ==

== Culturing Conditions ==
''Methanobrevibacter boviskoreani'' JH1T is a strict anaerobe and can be grown in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H2/CO2 (80:20, v/v). Growth of ''M. boviskoreani JH1T'' requires rumen fluid but does not need volatile fatty acids (VFAs). Upon plating, the strain forms colonies in 7-10 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .

== Antibiotic MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Puromycin
|
|-
|Nourseothricin
|
|-
|Neomycin
|
|-
|8-ADP
|
|-
|5-Fluorouracil
|
|-
|2-BES
|
|-
|5-FOA
|
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter millerae ZA-10T

2026-10-28T18:44:16Z

Sharik:

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexander, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|Adam Guss, Oak Ridge National Laboratory
|-
|MIC and Culturing Conditions by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|Sharik Khan, University of Illinois at Urbana-Champaign
Komal Aggrawal, University of Illinois at Urbana-Champaign
|}

== Genome ==
Single contig of 2,823,065 bp.

== DefenseFinder Results ==

== Methylome ==
{| class="wikitable"
| valign="top" |'''#6mA modified motifs'''
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#motif'''
| valign="top" |methylCounts
| valign="top" |totalCounts
| valign="top" |%motifsMethyl
|-
| valign="top" |'''CA(6mA)NNNNNNNTTG'''
| valign="top" |1905
| valign="top" |1944
| valign="top" |97.99
|-
| valign="top" |'''CRT(6mA)NNNNNNCCT'''
| valign="top" |428
| valign="top" |460
| valign="top" |93.04
|-
| valign="top" |'''(6mA)GGNNNNNNTAYG'''
| valign="top" |432
| valign="top" |460
| valign="top" |93.91
|-
| valign="top" |'''G(6mA)TC'''
| valign="top" |4913
| valign="top" |6856
| valign="top" |71.66
|-
| valign="top" |'''GCT(6mA)NNNNNNRTTA'''
| valign="top" |103
| valign="top" |106
| valign="top" |97.17
|-
| valign="top" |'''TA(6mA)YNNNNNNTAGC'''
| valign="top" |104
| valign="top" |106
| valign="top" |98.11
|-
| valign="top" |
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#4mC modified motifs'''
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#motif'''
| valign="top" |methylCounts
| valign="top" |totalCounts
| valign="top" |%motifsMethyl
|-
| valign="top" |'''TC(4mC)AT'''
| valign="top" |3383
| valign="top" |8313
| valign="top" |40.70
|}

== Methylating E. coli Strains ==
Two ''E. coli'' methylation strains, AG22054 (''dam-'') and AG22055 (''dam+''), expressing ''M. millerae ZA-10T'' methylases have been constructed by Adam Guss's group at the Oak Ridge National laboratory.

== Culturing Conditions ==
''M. millerae ZA-10T'' is a strict anaerobe and grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H2/CO2 (80:20, v/v). Growth of ''M. millerae ZA-10T'' requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 5-7 days when incubated at 37°C in Wolfe incubator filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .

== Antibiotic MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Puromycin
|
|-
|Nourseothricin
|
|-
|Neomycin
|
|-
|8-ADP
|
|-
|5-Fluorouracil
|
|-
|2-BES
|
|-
|5-FOA
|
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter millerae ZA-10T

2026-10-28T18:36:12Z

Sharik: /* Antibiotic MIC Tests */

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexander, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|Adam Guss, Oak Ridge National Laboratory
|-
|MIC and Culturing Conditions by:
|Sharik Khan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|Sharik Khan, University of Illinois at Urbana-Champaign
Komal Aggrawal, University of Illinois at Urbana-Champaign
|}

== Genome ==
Single contig of 2,823,065 bp.

== DefenseFinder Results ==

== Methylome ==
{| class="wikitable"
| valign="top" |'''#6mA modified motifs'''
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#motif'''
| valign="top" |methylCounts
| valign="top" |totalCounts
| valign="top" |%motifsMethyl
|-
| valign="top" |'''CA(6mA)NNNNNNNTTG'''
| valign="top" |1905
| valign="top" |1944
| valign="top" |97.99
|-
| valign="top" |'''CRT(6mA)NNNNNNCCT'''
| valign="top" |428
| valign="top" |460
| valign="top" |93.04
|-
| valign="top" |'''(6mA)GGNNNNNNTAYG'''
| valign="top" |432
| valign="top" |460
| valign="top" |93.91
|-
| valign="top" |'''G(6mA)TC'''
| valign="top" |4913
| valign="top" |6856
| valign="top" |71.66
|-
| valign="top" |'''GCT(6mA)NNNNNNRTTA'''
| valign="top" |103
| valign="top" |106
| valign="top" |97.17
|-
| valign="top" |'''TA(6mA)YNNNNNNTAGC'''
| valign="top" |104
| valign="top" |106
| valign="top" |98.11
|-
| valign="top" |
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#4mC modified motifs'''
| valign="top" |
| valign="top" |
| valign="top" |
|-
| valign="top" |'''#motif'''
| valign="top" |methylCounts
| valign="top" |totalCounts
| valign="top" |%motifsMethyl
|-
| valign="top" |'''TC(4mC)AT'''
| valign="top" |3383
| valign="top" |8313
| valign="top" |40.70
|}

== Methylating E. coli Strains ==
Two ''E. coli'' methylation strains, AG22054 (''dam-'') and AG22055 (''dam+''), expressing ''M. millerae ZA-10T'' methylases have been constructed by Adam Guss's group at the Oak Ridge National laboratory.

== Culturing Conditions ==
''M. millerae ZA-10T'' grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H2/CO2 (80:20, v/v). Growth of ''M. millerae ZA-10T'' requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 5-7 days when incubated at 37°C in Wolfe incubators filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .

== Antibiotic MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Puromycin
|
|-
|Nourseothricin
|
|-
|Neomycin
|
|-
|8-ADP
|
|-
|5-Fluorouracil
|
|-
|2-BES
|
|-
|5-FOA
|
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter olleyae 1H5-1PT

2026-10-28T18:13:45Z

Sharik: conditions

== Introduction ==

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|AgResearch, New Zealand
|-
|Sequenced by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Assembled by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Methylome called by:
|William G. Alexande, Oak Ridge National Laboratory
|-
|Methyltransferases cloned by:
|N/A
|-
|MIC and Culturing Conditions by:
|Glen Cronan, University of Illinois at Urbana-Champaign
|-
|Transformation by:
|Sharik Khan, University of Illinois at Urbana-Champaign
Komal Agrawal, University of Illinois at Urbana-Champaign
|}

== Genome ==

== DefenseFinder Results ==

== Methylome ==
No methylation motifs were detected in ''Methanobrevibacter olleyae'' 1H5-1PT.

== Methylating E. coli Strains ==
No ''E. coli'' methylation strain has been constructed for ''Methanobrevibacter olleyae'' 1H5-1PT.

== Culturing Conditions ==
''M. olleyae'' 1H5-1PT grows well in both RMO2-medium and MSM (''Methanobrevibacter smithii'' medium) topped with H2/CO2 (80:20, v/v). Growth of ''M. olleyae'' 1H5-1PT requires rumen fluid but does not need volatile fatty acids (VFAs), or sodium sulfide. The plating efficiency of the strain on MSM-agar plates is > 90% and colonies are visible in 4-5 days when incubated at 37°C in Wolfe incubators filled with a gas mixture of H2/CO2 (80:20) supplemented with 1000ppm H2S .

== MIC Tests ==
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|Puromycin
|
|-
|Nourseothricin
|
|-
|Neomycin
|
|-
|8-ADP
|
|-
|5-Fluorouracil
|
|-
|2-BES
|
|-
|5-FOA
|
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==

Methanobrevibacter boviskoreani JH1T

2026-10-28T17:29:04Z

Sharik: metadata

Methanobrevibacter olleyae 1H5-1PT

2026-10-28T17:18:28Z

Sharik: /* Global Methane Hub Metadata */

Methanobrevibacter millerae ZA-10T

2026-10-28T17:16:43Z

Sharik: matadata

Methanobrevibacter olleyae 1H5-1PT

2026-10-28T17:16:16Z

Sharik: metadata