Global Methane Hub Wikipedia - User contributions [en]

Methanobrevibacter olleyae YLM1

2026-10-30T15:55:04Z

Rodriguezcrj: /* Methylation pattern */ Updating methylome

== Introduction ==
''Methanobrevibacter olleyae'' YLM1 is a hydrogenotrophic methanogen isolated from the rumen of a lamb<ref>Skillman LC, Evans PN, Naylor GE, Morvan B, Jarvis GN, Joblin KN. 16S ribosomal DNA-directed PCR primers for ruminal methanogens and identification of methanogens colonising young lambs. Anaerobe. 2004 Oct;10(5):277-85. doi: [https://www.sciencedirect.com/science/article/pii/S1075996404000496?via%3Dihub 10.1016/j.anaerobe.2004.05.003]. </ref>. Unlike the type species of the genus ''Methanobrevibacter ruminantium'' M1<ref>Leahy SC, Kelly WJ, Altermann E, Ronimus RS, Yeoman CJ, Pacheco DM, Li D, Kong Z, McTavish S, Sang C, Lambie SC, Janssen PH, Dey D, Attwood GT. The genome sequence of the rumen methanogen ''Methanobrevibacter ruminantium'' reveals new possibilities for controlling ruminant methane emissions. PLoS One. 2010 Jan 28;5(1):e8926. doi: [https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0008926 10.1371/journal.pone.0008926].</ref>, YLM1 possesses the ''com''ADE genes, supporting the prediction that it can biosynthesize coenzyme M. It also possesses the ''mrt''BDGA operon encoding methyl-coenzyme M reductase II, which is absent from M1 and may contribute to differences in growth under varying hydrogen gas concentrations<ref name=":0">Kelly WJ, Li D, Lambie SC, Cox F, Attwood GT, Altermann E, Leahy SC. Draft Genome Sequence of the Rumen Methanogen ''Methanobrevibacter olleyae'' YLM1. Genome Announc. 2016 Apr 7;4(2):e00232-16. doi: [https://journals.asm.org/doi/10.1128/genomea.00232-16 10.1128/genomeA.00232-16].</ref>. These genomic features make YLM1 a useful strain for comparative studies of rumen methanogen physiology and the development of methane mitigation strategies. Its robust growth and high plating efficiency, observed in the Whitman Lab, make it particularly valuable for research supported by the Global Methane Hub.

== Global Methane Hub Metadata ==
{| class="wikitable"
|'''Origin:'''
|Lucidome Bio, NZ
|-
|'''Sequenced by:'''
|Plasmidsaurus, ONT + Illumina
|-
|'''Assembled by:'''
|Nana Shao
|-
|'''Methylome called by:'''
|William G. Alexander
|-
|'''Methyltransferases cloned by:'''
|Roy Rodriguez Carrero
|-
|'''MIC and Culturing conditions by:'''
|Nana Shao
|-
|'''Transformation by:'''
|
|}

== Genome ==
The draft genome of ''Methanobrevibacter olleyae'' YLM1 is 2,201,192 bp, with a GC content of 26.9% and 1,834 predicted protein-coding genes<ref name=":0" />. It contains one approximately 40-kb prophage, two CRISPR regions, and no identified plasmids. Notable features include the ''hdr''ABC genes encoding CoB-CoM heterodisulfide reductase subunits, the ''com''ADE genes involved in coenzyme M biosynthesis, the ''mrt''BDGA operon encoding methyl-coenzyme M reductase II, and 64 large adhesin-like protein genes. The GenBank accession number is [https://www.ncbi.nlm.nih.gov/nuccore/CP014265.1/ CP014265].

== Methylome ==

=== Methylation pattern ===
{| class="wikitable"
|+
!Methylated motif
!Percent methylated
|-
|GSNG(6mA)/GNSNG(6mA)
|98.35/95.91
|}

=== DefenseFinder Results ===
add here

=== Methylating ''E. coli'' ===
add here

== Culturing Conditions ==
''M. olleyae'' YLM1 is cultivated in [[Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM)|Modified ''Methanobrevibacter smithii'' and ''Methanosphaera stadtmanae'' medium (MMSM)]] at 37°C. To ensure good gas transfer to the media, tubes are incubated on their sides (or at a shallow angle so that the media does not contact the stoppers) with gentle shaking at 50 rpm and 207 kPa of H2/CO2 (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same pressure of H2/CO2 as an energy substrate for 3-7 days.

== Growth requirements ==
''M. olleyae'' YLM1 requires rumen fluid for robust growth in MMSM, whereas yeast extract and tryptone are individually dispensable under the conditions tested. Omission of rumen fluid markedly reduces growth, while omission of either yeast extract or tryptone still allows growth to OD600 values of ~1.4. The inability of filter-sterilized cysteine to support good growth indicates that is not used as a sulfur source and that sulfide from the breakdown of cysteine serves as a sulfur source for this strain.
{| class="wikitable"
|+
|[[File:Rumen fluid is required for M. olleyae YLM1 growth.jpg|thumb|Rumen fluid is required for ''M. olleyae'' YLM1 growth. Source: Dr. Nana Shao|center|333x333px]]
|[[File:Sulfide serves as a sulfur source for M. olleyae YLM1 .jpg|thumb|524x524px|Sulfide serves as a sulfur source for ''M. olleyae'' YLM1. Source: Nana Shao|center]]
|}

== Antimicrobial MIC Tests ==
[[File:Puromycin MIC determination in M. olleyae YLM1. Source- Dr. Nana Shao.jpg|thumb|Puromycin MIC determination in ''M. olleyae'' YLM1. Source: Dr. Nana Shao|left|290x290px]]'''Solid Medium'''

Puromycin susceptibility of ''M.'' ''olleyae'' YLM1 was assessed on MMSM solid medium under anaerobic conditions, with the bottle preparation and incubation performed as described in the [[Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM)|MMSM protocol]]. The absence of visible colonies was used as the endpoint. The apparent MIC was 10 µg/mL at an inoculum of 105 cells/plate and 15 µg/mL at 107 cells/plate. Below the MIC, confluent growth or numerous small colonies are observed on the agar surface. Large colonies are observed when fewer than 100 cells survive. When growth is inhibited but not completely suppressed, small colonies are observed.

'''Liquid Medium'''
[[File:Antimicrobial MIC Determination in M. olleyae YLM1 in Liquid Medium.jpg|left|thumb|290x290px|Antimicrobial MIC determination in ''M. olleyae'' YLM1 in Liquid MMSM]]
Puromycin and nourseothricin susceptibility was assessed in liquid MMSM at an initial inoculum density of 2×107 cells/mL, using the absence of a detectable increase in OD600 throughout the observation period as the endpoint.
{| class="wikitable"
|+
!Antibiotics
|MIC at 2×107 cells/mL
|Observation period
|-
|Puromycin
|30 µg/mL
|~650 h
|-
|nourseothricin
|>80 µg/mL
|~550 h
|}
Overall, the results demonstrated inoculum-dependent inhibition under the tested conditions, with higher apparent MICs at higher inoculum densities in both solid and liquid media.

Overall, puromycin showed inoculum-dependent inhibition on solid medium and prevented detectable growth in liquid medium, whereas nourseothricin delayed but did not prevent growth at the tested concentrations. For the lower inoculum of about 105 cells, the MIC represents the lowest concentration that completely inhibits growth of wild-type cells. For the higher inoculum of about 107 cells, the MIC represents the lowest concentration that completely inhibits growth of spontaneous resistance mutants.

== Protocols ==
add here

== Transformation Results ==
add here

== External Links ==
<references />

Methanocatella millerae SM9

2026-10-30T15:40:19Z

Rodriguezcrj: /* Methylome */ Updating methylome

== Introduction ==
''Methanocatella millerae'' SM9 (formerly ''Methanobrevibacter millerae'') was isolated from the rumen of a sheep fed a fresh forage diet<ref>Joblin KN. (1999) Ruminal acetogens and their potential to lower ruminant methane emissions. ''Australian Journal of Agricultural Research'' 50, 1307–1314. [https://connectsci.au/cp/article/50/8/1307/33473/Ruminal-acetogens-and-their-potential-to-lower https://doi.org/10.1071/AR99004]</ref>. Although initially identified as ''Methanobrevibacter'' sp. or ''Methanobrevibacter smithii'', SM9 shares 99% 16S rRNA gene sequence identity with the ''Methanocatella (Methanobrevibacter) millerae'' type strain ZA-10ᵀ (DSM 16643), supporting its assignment to this species<ref>Kelly WJ, Pacheco DM, Li D, Attwood GT, Altermann E, Leahy SC. The complete genome sequence of the rumen methanogen ''Methanobrevibacter millerae'' SM9. Stand Genomic Sci. 2016 Aug 17;11:49. doi: [https://link.springer.com/article/10.1186/s40793-016-0171-9 10.1186/s40793-016-0171-9].</ref>. Its robust growth and high plating efficiency make SM9 a strain of particular interest to the Global Methane Hub.

== Global Methane Hub Metadata ==
{| class="wikitable"
|'''Origin:'''
|Lucidome Bio, NZ
|-
|'''Sequenced by:'''
|Plasmidsaurus, ONT + Illumina
|-
|'''Assembled by:'''
|Nana Shao
|-
|'''Methylome called by:'''
|William G. Alexander
|-
|'''Methyltransferases cloned by:'''
|Roy Rodriguez Carrero
|-
|'''MIC and Culturing conditions by:'''
|Nana Shao
|-
|'''Transformation by:'''
|
|}

== Genome ==
add here

== Methylome ==

=== Methylation pattern ===
{| class="wikitable"
|+
!Methylated motif
!Percent methylated
|-
|C(5mC)TG
|92.68
|-
|(5mC)AGG
|77.20
|-
|GGN(5mC)C
|63.19
|}

=== DefenseFinder Results ===
add here

=== Methylating ''E. coli'' ===
add here

== Culturing Conditions ==
''M. millerae'' SM9 is cultivated in [[Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM)|Modified ''Methanobrevibacter smithii'' and ''Methanosphaera stadtmanae'' medium (MMSM)]] at 37°C. To ensure good gas transfer to the media, tubes are incubated on their sides (or at a shallow angle so that the media does not contact the stoppers) with gentle shaking at 50 rpm and 207 kPa of H2/CO2 (80:20, v/v) as an energy substrate for 2-4 days. This methanogen can also be cultivated on MMSM plates using 1.5% agar as a gelling agent with the same pressure of H2/CO2 as an energy substrate for 3-7 days.

== Growth requirements ==
''M. millerae'' SM9 requires rumen fluid, yeast extract, and tryptone for optimal growth in MMSM. Omission of rumen fluid markedly reduces growth, whereas omission of either yeast extract or tryptone results in a moderate reduction in growth yield. Either cysteine (3 mM; autoclaved or filter-sterilized) or sodium sulfide (Na₂S; 0.4-1 mM) supports robust growth as a sulfur source, with no clear preference between the two. Under these conditions, cultures reach a maximum OD600 of approximately ~1.3. No growth was observed with formate as the sole energy substrate under the conditions tested.
{| class="wikitable"
|+
|[[File:Rumen fluid, yeast extract and tryptone are all required for optimal growth of M. millerae SM9 .jpg|thumb|Rumen fluid, yeast extract and tryptone are all required for optimal growth of ''M. millerae'' SM9. Source: Dr. Nana Shao]]
|[[File:Sulfur source of M. millerae SM9.jpg|thumb|Sulfur source of ''M. millerae'' SM9. Source: Dr. Nana Shao]]
|[[File:Influence of Na2S concentration on M. millerae SM9 growth.jpg|thumb|Influence of Na2S concentration on ''M. millerae'' SM9 growth. Source: Dr. Nana Shao]]
|}

== Antimicrobial MIC Tests ==
[[File:Puromycin MIC determination in M. millerae SM9. Source- Dr. Nana Shao..jpg|thumb|246x246px|Puromycin MIC determination in ''M. millerae'' SM9. Source: Dr. Nana Shao|left]]'''Solid Medium'''

Puromycin susceptibility of ''M. millerae'' SM9 was assessed on MMSM solid medium under anaerobic conditions, with the bottle preparation and incubation performed as described in the [[Modified Methanobrevibacter smithii and Methanosphaera stadtmanae medium (MMSM)|MMSM protocol]]. The absence of visible colonies was used as the endpoint. The apparent MIC was 10 µg/mL at an inoculum of 105 cells/plate and 20 µg/mL at 107 cells/plate. Below the MIC, confluent growth or numerous small colonies are observed on the agar surface. Large colonies are observed when fewer than 100 cells survive. When growth is inhibited but not completely suppressed, small colonies are observed.

'''Liquid Medium'''

Puromycin and nourseothricin susceptibility was assessed in liquid MMSM at initial inoculum densities of 8×105 and 2×107 cells/mL, using the absence of a detectable increase in OD600 throughout the observation period as the endpoint.

{| class="wikitable"
|+
|-
!Antibiotics
|MIC at 8×105 cells/mL
|MIC at 2×107 cells/mL
|Observation period
|-
|Puromycin
|10 µg/mL
|20 µg/mL
|~700 h
|-
|nourseothricin
|40 µg/mL
|70 µg/mL
|~630 h
|}
{| class="wikitable"
|+
|[[File:Puromycin MIC determination in M. millerae SM9 in liquid MMSM.jpg|left|thumb|408x408px|Puromycin MIC determination in ''M. millerae'' SM9 in liquid MMSM. Source: Dr. Nana Shao]]
|[[File:Nourseothricin MIC determination in M. millerae SM9 in liquid MMSM.jpg|center|thumb|411x411px|Nourseothricin MIC determination in ''M. millerae'' SM9 in liquid MMSM. Source: Dr. Nana Shao]]
|}
Overall, the results demonstrated inoculum-dependent inhibition under the tested conditions, with higher apparent MICs at higher inoculum densities in both solid and liquid media. For the lower inoculum of about 105 cells, the MIC represents the lowest concentration that completely inhibits growth of wild-type cells. For the higher inoculum of about 107 cells, the MIC represents the lowest concentration that completely inhibits growth of spontaneous resistance mutants.

== Protocols ==
add here

== Transformation Results ==
add here

== External Links ==
<references />

Sharpea azabuensis KH1P5

2026-10-22T18:45:43Z

Rodriguezcrj: /* Introduction */ Added context for the EPS formation in Sharpea on commercial RCM and linked the page with more context on RCM.

[[File:Sabu tube.png|thumb|''S. azabuensis'' KH1P5 in liquid culture exhibiting its dense layer of EPS. Source: Dr. Roy Rodriguez Carrero|346x346px]]

== Introduction ==
'''Sharpea azabuensis KH1P5'''

Sharpea azabuensis KH1P5 was isolated from bovine rumen contents in NZ using KH medium which was supplemented with haemin and vitamin K [1]. KH1P5 ferments glucose, fructose, galactose, cellobiose, sucrose, lactose and raffinose with D-lactate as the main fermentation product together with small amounts of acetate, formate and ethanol [2]. It is a member of the family Coprobacillaceae.

Sharpea and Kandleria were identified as key bacteria associated with the S-type (Sharpea-enriched) bacterial community, one of the two low-CH4 community types in rumen samples from sheep that differed in the amount of CH4 formed per unit of feed consumed when these sheep were fed pelletized lucerne [3]. A metagenomic and metatranscriptomic study conducted by Kamke et al. [4] confirmed that the relative abundance of Sharpea spp. was greater in a group of low-CH4 emitting sheep than in their high-CH4 emitting flock mates. It was hypothesized that lactate produced by these bacteria is further converted to butyrate by Megasphaera spp., resulting in lower CH4 emissions.

The draft genome for KH1P5 (2,329,465 bp) was generated through the Hungate1000 project with JGI [1]. Resequencing gave a single chromosome of 2,465,508 bp.

LPSN: ''Sharpea azabuensis'' Morita ''et al.'' 2008

NCBI: Sharpea azabuensis.

GTDB: Sharpea azabuensis. KH1P5 groups with the type strain (ST18, DSM 18934) [5]

Risk group: 1

''[[Sharpea azabuensis]]'' KH1P5 is a high-interest microbe to the Global Methane Hub. It produces a thick, mucoidal layer of [[wikipedia:Extracellular_polymeric_substance|extracellular polymeric substance (EPS)]] that requires [[Sharpea azabuensis KH1P5#Protocols|removal via washing]] with chelating agents and detergent before work with the culture. It was noted that this dense layer of EPS was a result of the agar found in the commercially available [[Reinforced Clostridial Medium|RCM]]. Growing the organism in lab made RCM without agar eliminates the dense EPS layer.

== Global Methane Hub Metadata ==
{| class="wikitable"
|+
|Origin:
|New Zealand, Kelly Lab
|-
|Sequenced by:
|Sam Mahoney Kurpe
|-
|Assembled by:
|William G. Alexander
|-
|Methylome called by:
|William G. Alexander
|-
|Methyltransferases cloned by:
|Roy Rodriguez Carrero
|-
|MIC and Culturing Conditions by:
|Roy Rodriguez Carrero
|-
|Transformation by:
|Roy Rodriguez Carrero
|}

== Genome ==
[[File:S azabuensis circos.png|Circos plot of ''S. azabuensis'' KH1P5. Source: Dr. William G. Alexander|center|thumb]]

== DefenseFinder Results ==

== Methylome ==
While ''S. azabuensis'' KH1P5 possesses two putative Type II restriction endonucleases, no methylation is detectable in the isolate. Also, these two genes do not methylate in ''E. coli'' strains, suggesting that they're vestigial defense systems.

== Methylating E. coli Strains ==
Use a strain of ''E. coli'' that lacks dam, dcm, and hsdRMS, such as AG5589.

== Culturing Conditions ==
''S. azabuensis'' KH1P5 grows well in stock [[RCM]] at 37 °C.

== Antibiotic MIC Tests ==
[[File:Sabu MIC.png|left|thumb|MIC tests of ''S. azabuensis''. Source: Dr. Roy Rodriguez Carrero]]
{| class="wikitable"
!antibiotic
!concentration (ug/mL)
|-
|chloramphenicol
|0.38
|-
|erythromycin
|1
|-
|kanamycin
|insensitive
|-
|spectinomycin
|96
|-
|tetracycline
|0.23
|}

== Protocols ==

* [[Removal of extracellular material via chelation washing]]

== Transformation Results ==

== External Links ==
[1] Seshadri R, Leahy SC, Attwood GT, Teh KH, Lambie SC, Cookson AL, Eloe-Fadrosh EA, Pavlopoulos GA, Hadjithomas M, Varghese NJ, Paez-Espino D; Hungate1000 project collaborators; Perry R, Henderson G, Creevey CJ, Terrapon N, Lapebie P, Drula E, Lombard V, Rubin E, Kyrpides NC, Henrissat B, Woyke T, Ivanova NN, Kelly WJ. 2019. Cultivation and sequencing of rumen microbiome members from the Hungate1000 Collection. Nature Biotechnol 36: 359-367. doi: 10.1038/nbt.4110.

[2] Kumar S, Treloar BP, Teh KH, McKenzie CM, Henderson G, Attwood GT, Waters SM, Patchett ML, Janssen PH. 2018. Sharpea and Kandleria are lactic acid producing rumen bacteria that do not change their fermentation products when co-cultured with a methanogen. Anaerobe 54: 31-38. doi: 10.1016/j.anaerobe.2018.07.008.

[3] Kittelmann S, Pinares-Patiño CS, Seedorf H, Kirk MR, Ganesh S, McEwan JC, Janssen PH. 2014. Two different bacterial community types are linked with the low-methane emission trait in sheep. PLoS One 9: e103171. doi: 10.1371/journal.pone.0103171.

[4] Kamke J, Kittelmann S, Soni P, Li Y, Tavendale M, Ganesh S, Janssen PH, Shi W, Froula J, Rubin EM, Attwood GT. 2016. Rumen metagenome and metatranscriptome analyses of low methane yield sheep reveals a Sharpea-enriched microbiome characterised by lactic acid formation and utilisation. Microbiome 4: 56. doi: 10.1186/s40168-016-0201-2.

[5] Morita H, Shiratori C, Murakami M, Takami H, Toh H, Kato Y, Nakajima F, Takagi M, Akita H, Masaoka T, Hattori M. 2008. Sharpea azabuensis gen. nov., sp. nov., a Gram-positive, strictly anaerobic bacterium isolated from the faeces of thoroughbred horses. Int J Syst Evol Microbiol 58: 2682-2686 doi: 10.1099/ijs.0.65543-0.

Reinforced Clostridial Medium

2026-10-22T18:41:41Z

Rodriguezcrj: Added notes on the effects that the agar in commercial RCM has on EPS formation.

== Description ==
Reinforced Clostridial Medium (RCM) is a common rich undefined microbiological medium for the anaerobic culturing of microbes.

== Recipe ==
{| class="wikitable"
|+
!Compound
!g/L
|-
|Yeast Extract
|13
|-
|Peptone
|10
|-
|Soluble Starch
|1
|-
|Glucose
|5
|-
|Cysteine HCl
|0.5
|-
|Sodium Chloride
|5
|-
|Sodium Acetate
|3
|-
|Agar
|0.5
|}
Note: some suppliers may replace up to 10 g of yeast extract with meat extract

=== Directions ===
Suspend 38 g of RCM in 1 liter of distilled water. Boil to dissolve solids completely, aliquot, and sterilize by autoclaving at 121 °C for 15 minutes.

== Notes ==
[[File:Cell pellet formation of Megasphaera elsdenii strain AG22635 in RCM variant.png|thumb|Cell pellet of Megasphaera elsdenii strain AG22635 after a 10-minute 5000 x g centrifuge spin. The culture on the left was grown in commercial RCM. The culture on the right was grown in lab-made RCM without agar.]]
Store bought RCM contains 0.5 g/L agar to mitigate oxygen permeability into the medium. The addition of this agar can have a compounding effect on the formation of [[wikipedia:Extracellular_polymeric_substance|extracellular polymeric substances (EPS)]] in some species like [[Sharpea azabuensis KH1P5|Sharpea azubuensis]] and [[Megasphaera elsdenii T81|Megasphaera elsdeni]]. This EPS generation makes the formation of cell pellets difficult and can affect the procedure of making competent cells. It's recommended to make your RCM "in house" without agar to avoid this complication.

<gallery>
</gallery>

== Sources ==

* [https://documents.thermofisher.com/TFS-Assets/MBD/Specification-Sheets/BT-SPEC-0099.pdf ThermoFisher CM0149] (Used by Guss Lab)

File:Cell pellet formation of Megasphaera elsdenii strain AG22635 in RCM variant.png

2026-10-22T18:36:03Z

Rodriguezcrj:

Cell pellet of Megasphaera elsdenii strain AG22635 after a 10-minute 5000 x g centrifuge spin. The culture on the left was grown in commercial RCM. The culture on the right was grown in lab-made RCM without agar.

File:Cell pellet formation of Megasphaera elsdenii strain AG22635 in RCM variant.png

2026-10-22T18:32:15Z

Rodriguezcrj:

Cell pellet of Megasphaera elsdenii strain AG22635 after a 10-minute 5000 xg centrifuge spin. The culture on the left was grown in commercial RCM. The culture on the right was grown in lab-made RCM without agar.

Ruminococcus albus 7

2026-09-30T15:31:24Z

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |G(6mA)TC | |- |GCGC(6mA)G | |- |CAG(6mA)KC | |- |CTYR(6mA)G | |- |C(6mA)GNNNNNNNTTYG/ CRA(6mA)NNNNNNNCTG | |- |G(6mA)STC | |- |GAGG(6mA)G | |- |G(5mC)WGC | |- |GGN(5mC)C | |- |CTC(4mC)TC | |}"

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|G(6mA)TC
|
|-
|GCGC(6mA)G
|
|-
|CAG(6mA)KC
|
|-
|CTYR(6mA)G
|
|-
|C(6mA)GNNNNNNNTTYG/

CRA(6mA)NNNNNNNCTG
|
|-
|G(6mA)STC
|
|-
|GAGG(6mA)G
|
|-
|G(5mC)WGC
|
|-
|GGN(5mC)C
|
|-
|CTC(4mC)TC
|
|}

Ruminococcus albus

2026-09-30T15:27:51Z

Rodriguezcrj: Created page with "== Global Methane Hub Strains == ''Ruminococcus albus'' 7"

== Global Methane Hub Strains ==
[[Ruminococcus albus 7|''Ruminococcus albus'' 7]]

Methanobrevibacter millerae ZA-10

2026-09-30T15:25:16Z

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |CRT(6mA)NNNNNNCCT/ (6mA)GGNNNNNNTAYG | |- |GCT(6mA)NNNNNNRTTA/ TA(6mA)YNNNNNNTAGC | |- |CA(6mA)NNNNNNNTTG | |- |G(6mA)TC | |}"

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|CRT(6mA)NNNNNNCCT/

(6mA)GGNNNNNNTAYG
|
|-
|GCT(6mA)NNNNNNRTTA/

TA(6mA)YNNNNNNTAGC
|
|-
|CA(6mA)NNNNNNNTTG
|
|-
|G(6mA)TC
|
|}

Methanobrevibacter millerae

2026-09-30T15:14:36Z

Rodriguezcrj: Created page with "== Global Methane Hub Strains == ''Methanobrevibacter millerae'' ZA-10"

== Global Methane Hub Strains ==
[[Methanobrevibacter millerae ZA-10|''Methanobrevibacter millerae'' ZA-10]]

Prevotella bryantii B14

2026-09-30T15:09:46Z

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |GTC(6mA)NNNNNRTC/G(6mA)YNNNNNTGAC |75.00%/80.51% |- |GTHN(6mA)NCC |76.99% |- |GTC(6mA)G |71.12% |- |A(6mA)CNNNNNGGG/C(4mC)CNNNNNGTT |82.78%/ |- |GG(5mC)GT |82.25% |- |A(5mC)GCC |81.20% |}"

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|GTC(6mA)NNNNNRTC/G(6mA)YNNNNNTGAC
|75.00%/80.51%
|-
|GTHN(6mA)NCC
|76.99%
|-
|GTC(6mA)G
|71.12%
|-
|A(6mA)CNNNNNGGG/C(4mC)CNNNNNGTT
|82.78%/
|-
|GG(5mC)GT
|82.25%
|-
|A(5mC)GCC
|81.20%
|}

Prevotella bryantii

2026-09-30T14:57:34Z

Rodriguezcrj: /* Global Methane Hub Strains */

== Global Methane Hub Strains ==
[[Prevotella bryantii C21a|''Prevotella bryantii'' C21a]]

[[Prevotella bryantii B14|''Prevotella bryantii'' B14]]

Butyrivibrio hungatei MB2003

2026-09-30T14:49:07Z

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ !Motif !Percent methylated |- |C(6mA)CNNNNNNTGTT/ AAC(6mA)NNNNNNGTG |94.54%/94.83% |- |ATTA(6mA)T |97.76% |}"

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|C(6mA)CNNNNNNTGTT/

AAC(6mA)NNNNNNGTG
|94.54%/94.83%
|-
|ATTA(6mA)T
|97.76%
|}

Butyrivibrio hungatei

2026-09-30T14:44:20Z

Rodriguezcrj: Created page with "== Global Methane Hub Strains == ''Butyrivibrio hungatei'' MB2003"

== Global Methane Hub Strains ==
[[Butyrivibrio hungatei MB2003|''Butyrivibrio hungatei'' MB2003]]

Prevotella bryantii C21a

2026-09-30T14:35:25Z

Rodriguezcrj: /* Methylome */

== Methylome ==
{| class="wikitable"
|+
|'''Motif'''
!Percent methylated
|-
|ACGC(6mA)B
|98.66%
|-
|CAAG(6mA)T
|96.36%
|-
|
|
|}

Prevotella bryantii C21a

2026-09-30T14:17:20Z

Rodriguezcrj: Created page with "== Methylome == {| class="wikitable" |+ |'''Isolate Motif''' !Percent methylated |- |ACGC(6mA)B |98.66% |- |CAAG(6mA)T |96.36% |- | | |}"

== Methylome ==
{| class="wikitable"
|+
|'''Isolate Motif'''
!Percent methylated
|-
|ACGC(6mA)B
|98.66%
|-
|CAAG(6mA)T
|96.36%
|-
|
|
|}

Prevotella bryantii

2026-09-30T14:15:47Z

Rodriguezcrj: Created page with "== Global Methane Hub Strains == Prevotella bryantii C21a"

== Global Methane Hub Strains ==
[[Prevotella bryantii C21a]]

Prevotella ruminicola 23T

2026-09-30T13:36:52Z

Rodriguezcrj: /* Methylome */

== Description ==

== Genome ==

== Methylome ==
{| class="wikitable"
|+
!Motif
!Percent methylated
|-
|G(6mA)TC
|95.98
|-
|CYC(6mA)NNNNNNNTCG/CG(6mA)NNNNNNNTGRG
|86.34/95.59
|-
|GR(5mC)GYC
|95.15
|-
|GG(5mC)C
|85.78
|-
|AG(5mC)TC
|84.75
|-
|GAG(5mC)T
|77.28
|}